Pulmonary expression of HIF-1α and its relationship with GRP78 in the pathogenesis of hepatopulmonary syndrome in rats

Xu Jiong Li, Hui Ying Zhang, Xiao Xia Tian, Yun Xia Cheng, Li Meng, Li Na Lai, Z. Zhao, De Wu Han, Ji Cheng

Research output: Contribution to journalArticle

Abstract

Objective: To explore the role of HIF-1α in the pathogenesis of hepatopulmonary syndrome(HPS) and its relationship with GRP78. Methods: The HPS model in rats was induced by multiple pathogenic factor. The samples were assessed by using Western blotting analysis for HIF-lα, GRP78 and VEGF164. The expressions of VEGFR-2 and CD105 were observed by using immunohistochemical staining. Results: The protein level of HIF-1α was significantly increased in HPS group at week 8 compared with that at week 4 and 6 groups and corresponding normal control groups. With the development of HPS, protein level of GRP78 was gradually increased at each time point significantly and reached the highest level at week 8; protein level of VEGF164 showed a similar change with GRP78, but the peak was at week 6. Immunohistochemical results showed that the protein expressions of VEGFR-2 and CD105 were gradually increased in lung tissue as HPS progressed. The protein level of GRP78 was positively correlated with HIF-1α, VEGF164, VEGFR-2 and CD105, respectively (P

Original languageEnglish
JournalJournal of Xi'an Jiaotong University (Medical Sciences)
Volume37
Issue number4
DOIs
Publication statusPublished - Jul 5 2016

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Keywords

  • 78kD glucose-regulated protein
  • Hepatopulmonary syndrome
  • Hypoxia-inducible factor-1α
  • Liver cirrhosis
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Medicine(all)
  • Clinical Biochemistry

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