P2 purinoceptors account for the non-nitrergic NANC relaxation in the rat ileum

R. Benkó, Sarolta Undi, Matyas Wolf, Klara Magyar, Zsuzsanna Tóvölgyi, Zoltan Rumbus, L. Barthó

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The transmitters involved in the non-nitrergic component of the non-adrenergic, non-cholinergic (NANC) inhibitory response of the rat small intestinal longitudinal muscle to electrical field stimulation of its nerves is a matter of controversy. The present study is the first one to utilise a combination of a nitric oxide synthase inhibitor and a P2 purinoceptor antagonist for studying this response. We found that the P 2 purinoceptor antagonist pyridoxalphosphate-6-azophenyl-2′, 4′-disulphonic acid (PPADS; 5×10-5 M) abolished the non-nitrergic NANC relaxation to electrical field stimulation (10 Hz). PPADS alone provided a significant, moderate inhibitory action. PPADS specifically inhibited relaxations due to exogenous adenosine 5′-triphosphate (ATP) or α,β-methylene ATP. The guanylate cyclase blocker 1H-[1,2,4] oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; 10-6 M) did not add to the inhibitory action of NG-nitro-L-arginine on field stimulation-induced relaxation. ODQ abolished the relaxant effect of the nitric oxide donors nitroglycerin or sodium nitroprusside. These data indicate that: (1) nitric oxide and ATP fully account for the field stimulation-induced relaxation in the rat ileal strip under the experimental conditions of this study, and (2) no ODQ-sensitive guanylate cyclase-mediated mechanism is involved in the non-nitrergic component of the NANC relaxation.

Original languageEnglish
Pages (from-to)319-324
Number of pages6
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume373
Issue number4
DOIs
Publication statusPublished - Jul 2006

Fingerprint

Purinergic P2 Receptors
Ileum
Adenosine Triphosphate
Guanylate Cyclase
Electric Stimulation
Purinergic P2 Receptor Antagonists
Purinergic Antagonists
Nitric Oxide Donors
Nitroarginine
Nitroglycerin
Nitroprusside
Nitric Oxide Synthase
Nitric Oxide
Muscles
Acids
pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid

Keywords

  • NANC relaxation
  • Nitric oxide
  • P purinoceptors
  • Purinergic nerves
  • Small intestine (rat)

ASJC Scopus subject areas

  • Pharmacology

Cite this

P2 purinoceptors account for the non-nitrergic NANC relaxation in the rat ileum. / Benkó, R.; Undi, Sarolta; Wolf, Matyas; Magyar, Klara; Tóvölgyi, Zsuzsanna; Rumbus, Zoltan; Barthó, L.

In: Naunyn-Schmiedeberg's Archives of Pharmacology, Vol. 373, No. 4, 07.2006, p. 319-324.

Research output: Contribution to journalArticle

Benkó, R. ; Undi, Sarolta ; Wolf, Matyas ; Magyar, Klara ; Tóvölgyi, Zsuzsanna ; Rumbus, Zoltan ; Barthó, L. / P2 purinoceptors account for the non-nitrergic NANC relaxation in the rat ileum. In: Naunyn-Schmiedeberg's Archives of Pharmacology. 2006 ; Vol. 373, No. 4. pp. 319-324.
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