PSA-NCAM in postnatally generated immature neurons of the olfactory bulb: A crucial role in regulating p75 expression and cell survival

Eduardo Gascon, Laszlo Vutskits, Benoit Jenny, Pascale Durbec, J. Kiss

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

In the mammalian brain, ongoing neurogenesis via the rostral migratory stream (RMS) maintains neuronal replacement in the olfactory bulb throughout life. Mechanisms that regulate the final number of new neurons in this system include proliferation, migration and apoptosis. Here we show that the polysialylated isoforms of the neural cell adhesion molecule (PSA-NCAM) act as a pro-survival molecule in immature newborn neurons. Confocal microscopic analysis revealed a threefold increase in TUNEL-positive cells in the subventricular zone (SVZ) and the RMS of transgenic animals lacking the gene encoding NCAM (NCAM-/-), as compared with wild types. The enhanced apoptotic cell death occurred specifically in the population of mCD24-positive newborn neurons, but not in GFAP-positive astrocytes. Using in vitro cultures of purified SVZ-derived neurons, we demonstrate that the loss or inactivation of PSA on NCAM, as well as the deletion of NCAM, lead to reduced survival in response to neurotrophins including BDNF and NGF. These changes in cell survival are accompanied by an upregulation of p75 neurotrophin receptor expression in vitro as well as in vivo. Furthermore, the negative effects of PSA-NCAM inactivation on cell survival could be prevented by the pharmacological blockade of the p75 receptor-signaling pathway. We propose that PSA-NCAM may promote survival by controlling the expression of the p75 receptor in developing neurons.

Original languageEnglish
Pages (from-to)1181-1190
Number of pages10
JournalDevelopment
Volume134
Issue number6
DOIs
Publication statusPublished - Mar 2007

Fingerprint

Neural Cell Adhesion Molecules
Olfactory Bulb
Cell Survival
Protein Isoforms
Neurons
Lateral Ventricles
Nerve Growth Factor Receptor
Genetically Modified Animals
Brain-Derived Neurotrophic Factor
Neurogenesis
In Situ Nick-End Labeling
Nerve Growth Factors
Nerve Growth Factor
Astrocytes
Cell Death
Up-Regulation
Pharmacology
Apoptosis
Brain
Population

Keywords

  • Neurogenesis
  • Neuronal survival
  • Neurotrophin
  • Olfactory bulb
  • p75 (Ngfr)
  • PSA-NCAM

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

PSA-NCAM in postnatally generated immature neurons of the olfactory bulb : A crucial role in regulating p75 expression and cell survival. / Gascon, Eduardo; Vutskits, Laszlo; Jenny, Benoit; Durbec, Pascale; Kiss, J.

In: Development, Vol. 134, No. 6, 03.2007, p. 1181-1190.

Research output: Contribution to journalArticle

Gascon, Eduardo ; Vutskits, Laszlo ; Jenny, Benoit ; Durbec, Pascale ; Kiss, J. / PSA-NCAM in postnatally generated immature neurons of the olfactory bulb : A crucial role in regulating p75 expression and cell survival. In: Development. 2007 ; Vol. 134, No. 6. pp. 1181-1190.
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