PrP fragment 106-126 is toxic to cerebral endothelial cells expressing PrP(C)

M. Deli, Suehiro Sakaguchi, Ryota Nakaoke, C. Ábrahám, Hideaki Takahata, Juraj Kopaček, Kazuto Shigematsu, Shigeru Katamine, Masami Niwa

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A hydrophobic, fibrillogenic peptide fragment of human prion protein (PrP106-126) had in vitro toxicity to neurons expressing cellular prion protein (PrP(C)). In this study, we proved that primary cultures of mouse cerebral endothelial cells (MCEC) express PrP(C). Incubation of MCEC with PrP106-126 (25-200 μM) caused a dose-dependent toxicity assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase release, bis-benzimide staining for nuclear morphology, and trypan blue exclusion test. Pentosan polysulphate (50-100 μg/ml), a drug effective in scrapie prophylaxis, dose-dependently attenuated the injury. MCEC cultures from mice homogenous for the disrupted PrP gene were resistant to the toxicity of PrP106-126. In conclusion, cerebral endothelium expressing PrP(C) may be directly damaged during spongiform encephalopathies. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish
Pages (from-to)3931-3936
Number of pages6
JournalNeuroReport
Volume11
Issue number17
Publication statusPublished - Nov 27 2000

Fingerprint

Poisons
Endothelial Cells
Pentosan Sulfuric Polyester
Scrapie
Peptide Fragments
Trypan Blue
Brain Diseases
Protein C
L-Lactate Dehydrogenase
Endothelium
Cell Culture Techniques
Staining and Labeling
Neurons
Wounds and Injuries
Pharmaceutical Preparations
Genes
Prion Proteins

Keywords

  • Cellular prion protein
  • Cerebral endothelial cells
  • Pentosan polysulphate
  • PrP fragment 106-126
  • Toxicity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Deli, M., Sakaguchi, S., Nakaoke, R., Ábrahám, C., Takahata, H., Kopaček, J., ... Niwa, M. (2000). PrP fragment 106-126 is toxic to cerebral endothelial cells expressing PrP(C). NeuroReport, 11(17), 3931-3936.

PrP fragment 106-126 is toxic to cerebral endothelial cells expressing PrP(C). / Deli, M.; Sakaguchi, Suehiro; Nakaoke, Ryota; Ábrahám, C.; Takahata, Hideaki; Kopaček, Juraj; Shigematsu, Kazuto; Katamine, Shigeru; Niwa, Masami.

In: NeuroReport, Vol. 11, No. 17, 27.11.2000, p. 3931-3936.

Research output: Contribution to journalArticle

Deli, M, Sakaguchi, S, Nakaoke, R, Ábrahám, C, Takahata, H, Kopaček, J, Shigematsu, K, Katamine, S & Niwa, M 2000, 'PrP fragment 106-126 is toxic to cerebral endothelial cells expressing PrP(C)', NeuroReport, vol. 11, no. 17, pp. 3931-3936.
Deli M, Sakaguchi S, Nakaoke R, Ábrahám C, Takahata H, Kopaček J et al. PrP fragment 106-126 is toxic to cerebral endothelial cells expressing PrP(C). NeuroReport. 2000 Nov 27;11(17):3931-3936.
Deli, M. ; Sakaguchi, Suehiro ; Nakaoke, Ryota ; Ábrahám, C. ; Takahata, Hideaki ; Kopaček, Juraj ; Shigematsu, Kazuto ; Katamine, Shigeru ; Niwa, Masami. / PrP fragment 106-126 is toxic to cerebral endothelial cells expressing PrP(C). In: NeuroReport. 2000 ; Vol. 11, No. 17. pp. 3931-3936.
@article{cce91a26cfea4b0d9f1ccf63cb7abf18,
title = "PrP fragment 106-126 is toxic to cerebral endothelial cells expressing PrP(C)",
abstract = "A hydrophobic, fibrillogenic peptide fragment of human prion protein (PrP106-126) had in vitro toxicity to neurons expressing cellular prion protein (PrP(C)). In this study, we proved that primary cultures of mouse cerebral endothelial cells (MCEC) express PrP(C). Incubation of MCEC with PrP106-126 (25-200 μM) caused a dose-dependent toxicity assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase release, bis-benzimide staining for nuclear morphology, and trypan blue exclusion test. Pentosan polysulphate (50-100 μg/ml), a drug effective in scrapie prophylaxis, dose-dependently attenuated the injury. MCEC cultures from mice homogenous for the disrupted PrP gene were resistant to the toxicity of PrP106-126. In conclusion, cerebral endothelium expressing PrP(C) may be directly damaged during spongiform encephalopathies. (C) 2000 Lippincott Williams and Wilkins.",
keywords = "Cellular prion protein, Cerebral endothelial cells, Pentosan polysulphate, PrP fragment 106-126, Toxicity",
author = "M. Deli and Suehiro Sakaguchi and Ryota Nakaoke and C. {\'A}brah{\'a}m and Hideaki Takahata and Juraj Kopaček and Kazuto Shigematsu and Shigeru Katamine and Masami Niwa",
year = "2000",
month = "11",
day = "27",
language = "English",
volume = "11",
pages = "3931--3936",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "17",

}

TY - JOUR

T1 - PrP fragment 106-126 is toxic to cerebral endothelial cells expressing PrP(C)

AU - Deli, M.

AU - Sakaguchi, Suehiro

AU - Nakaoke, Ryota

AU - Ábrahám, C.

AU - Takahata, Hideaki

AU - Kopaček, Juraj

AU - Shigematsu, Kazuto

AU - Katamine, Shigeru

AU - Niwa, Masami

PY - 2000/11/27

Y1 - 2000/11/27

N2 - A hydrophobic, fibrillogenic peptide fragment of human prion protein (PrP106-126) had in vitro toxicity to neurons expressing cellular prion protein (PrP(C)). In this study, we proved that primary cultures of mouse cerebral endothelial cells (MCEC) express PrP(C). Incubation of MCEC with PrP106-126 (25-200 μM) caused a dose-dependent toxicity assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase release, bis-benzimide staining for nuclear morphology, and trypan blue exclusion test. Pentosan polysulphate (50-100 μg/ml), a drug effective in scrapie prophylaxis, dose-dependently attenuated the injury. MCEC cultures from mice homogenous for the disrupted PrP gene were resistant to the toxicity of PrP106-126. In conclusion, cerebral endothelium expressing PrP(C) may be directly damaged during spongiform encephalopathies. (C) 2000 Lippincott Williams and Wilkins.

AB - A hydrophobic, fibrillogenic peptide fragment of human prion protein (PrP106-126) had in vitro toxicity to neurons expressing cellular prion protein (PrP(C)). In this study, we proved that primary cultures of mouse cerebral endothelial cells (MCEC) express PrP(C). Incubation of MCEC with PrP106-126 (25-200 μM) caused a dose-dependent toxicity assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase release, bis-benzimide staining for nuclear morphology, and trypan blue exclusion test. Pentosan polysulphate (50-100 μg/ml), a drug effective in scrapie prophylaxis, dose-dependently attenuated the injury. MCEC cultures from mice homogenous for the disrupted PrP gene were resistant to the toxicity of PrP106-126. In conclusion, cerebral endothelium expressing PrP(C) may be directly damaged during spongiform encephalopathies. (C) 2000 Lippincott Williams and Wilkins.

KW - Cellular prion protein

KW - Cerebral endothelial cells

KW - Pentosan polysulphate

KW - PrP fragment 106-126

KW - Toxicity

UR - http://www.scopus.com/inward/record.url?scp=0034722644&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034722644&partnerID=8YFLogxK

M3 - Article

VL - 11

SP - 3931

EP - 3936

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 17

ER -