Protochlorophyllide-NADP+ and protochlorophyllide-NADPH complexes and their regeneration after flash illumination in leaves and etioplast membranes of dark-grown wheat

Fabrice Franck, Barbara Bereza, Béla Böddi

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The fast (1 min) regeneration process of the photoactive Pchlide forms after a light flash was studied in etiolated wheat leaves, and this process was simulated in vitro by incubating etioplast inner membranes of wheat with excess NADPH or NADP+. The 77 K fluorescence spectra were recorded after flash illumination, dark incubation and a subsequent flash illumination of the samples. A non-photoactive Pchlide form with an emission maximum at 650 nm was transiently detected in leaves during regeneration of a photoactive Pchlide form with an emission maximum at 654 nm. Gaussian deconvolution of fluorescence spectra of isolated membranes showed that this 650 nm form appeared in conditions of excess NADP+, as suggested in previous studies. Additionally a Pchlide form emitting at 638.5 nm was detected in the same conditions. The analysis of the spectra of leaves at different times after a flash indicated that these two non-photoactive forms are involved as intermediates in the regeneration of photoactive Pchlide. This regeneration is in correlation with the production of the Chlide form emitting at 676 nm. The results demonstrate that, in vivo, part of the NADPH:protochlorophyllide oxidoreductase is reloading with nonphotoactive Pchlide on a fast time-scale and that the 676 nm Chlide form is the released product of the phototransformation in this process.

Original languageEnglish
Pages (from-to)53-61
Number of pages9
JournalPhotosynthesis research
Volume59
Issue number1
DOIs
Publication statusPublished - Jan 1 1999

Keywords

  • Fluorescence
  • NADPH:protochlorophyllide oxidoreductase
  • POR
  • Phototransformation
  • Protochlorophyllide

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

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