Protochlorophyllide forms in non‐greening epicotyls of dark‐grown pea (Pisum sativum)

Béla Böddi, Birgitta McEwen, Margareta Ryberg, Christer Sundqvist

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Abstract

Low‐temperature fluorescence emission spectra of 6.5‐day‐old dark‐grown epicotyls of pea (Pisum sativum) revealed the presence of protochlorophyll(ide). The upper part of the epicotyl contained 30% of the protochlorophyll(ide) content per fresh weight found in pea leaves, whereas the lower part contained 3%. Three discrete spectral forms of protochlorophyll(ide) were clearly distinguished after Gaussian deconvolution of fluorescence excitation and emission spectra. Adding the satellite bands of the Qy0‐0 transitions (the emission vibrational (Emv) bands with correlated amplitudes, gave the following delineation: Ex439–Em629–Emv684, Ex447–Em636–Emv700 and Ex456–Em650–Emv728. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE) followed by immunodetection of whole tissue extracts of the epicotyl indicated the presence of NADPH‐protochlorophyllide oxidoreductase (EC 1.3.1.33). Electron micrographs showed prolamellar bodies in at most 11 % of the plastid profiles of the epicotyl cells. These prolamellar bodies were smaller, and many of them showed less regular structure than those of the leaves. Taken together, the results indicate that the protochlorophyll(ide) in epicotyls is arranged in a different way than in leaves.

Original languageEnglish
Pages (from-to)160-170
Number of pages11
JournalPhysiologia Plantarum
Volume92
Issue number1
DOIs
Publication statusPublished - Sep 1994

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Keywords

  • Epicotyl
  • Gaussian component
  • NADPH‐protochlorophyllide oxidoreductase
  • Pisum sativum
  • etioplast
  • fluorescence emission spectra
  • fluorescence excitation spectra
  • prolamellar body
  • prothylakoid
  • protochlorophyllide
  • spectral forms

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

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