Protochlorophyll forms with different molecular arrangements.

B. Böddi, J. Soós, F. Láng

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Spectral properties of protochlorophyll ()PChl) forms were investigated in solid-film model systems by absorption fluorescence and circular dichroism (CD) spectroscopy. The solid films were prepared from diethyl ether solution of PChl on a cover glass surface by evaporation of the solvent. After preparation the films usually showed an absoprtion maximum at 635 nm or in some cases at 640 nm. The PChl form with 635 nm absorption maximum had no CD signal, whilst the films with absorption maximum at 640 nm gave an intense negative CD band at about 640 nm and a positive one at 668 nm. The treatment of the films with ammonia or acetone vapour resulted in a red shift of the absorption maximum from 635 nm or 640 nm to 650 nm. The study of the CD spectra of the films with different PChl forms showed that, depending on the treatment, forms of PChl with similar absorption and fluorescence spectra, but with opposite CD signals, can exist. It is suggested that the differences of the CD spectra are mainly due to different arrangements of the aggregates.

Original languageEnglish
Pages (from-to)158-165
Number of pages8
JournalBBA - General Subjects
Volume593
Issue number1
Publication statusPublished - Nov 5 1980

Fingerprint

Circular Dichroism
Dichroism
Fluorescence
Circular dichroism spectroscopy
Acetone
Ammonia
Ether
Glass
protochlorophyll
Spectrum Analysis
Evaporation
Vapors

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Protochlorophyll forms with different molecular arrangements. / Böddi, B.; Soós, J.; Láng, F.

In: BBA - General Subjects, Vol. 593, No. 1, 05.11.1980, p. 158-165.

Research output: Contribution to journalArticle

Böddi, B, Soós, J & Láng, F 1980, 'Protochlorophyll forms with different molecular arrangements.', BBA - General Subjects, vol. 593, no. 1, pp. 158-165.
Böddi, B. ; Soós, J. ; Láng, F. / Protochlorophyll forms with different molecular arrangements. In: BBA - General Subjects. 1980 ; Vol. 593, No. 1. pp. 158-165.
@article{0a795e4d16284b40914d086211ee89b1,
title = "Protochlorophyll forms with different molecular arrangements.",
abstract = "Spectral properties of protochlorophyll ()PChl) forms were investigated in solid-film model systems by absorption fluorescence and circular dichroism (CD) spectroscopy. The solid films were prepared from diethyl ether solution of PChl on a cover glass surface by evaporation of the solvent. After preparation the films usually showed an absoprtion maximum at 635 nm or in some cases at 640 nm. The PChl form with 635 nm absorption maximum had no CD signal, whilst the films with absorption maximum at 640 nm gave an intense negative CD band at about 640 nm and a positive one at 668 nm. The treatment of the films with ammonia or acetone vapour resulted in a red shift of the absorption maximum from 635 nm or 640 nm to 650 nm. The study of the CD spectra of the films with different PChl forms showed that, depending on the treatment, forms of PChl with similar absorption and fluorescence spectra, but with opposite CD signals, can exist. It is suggested that the differences of the CD spectra are mainly due to different arrangements of the aggregates.",
author = "B. B{\"o}ddi and J. So{\'o}s and F. L{\'a}ng",
year = "1980",
month = "11",
day = "5",
language = "English",
volume = "593",
pages = "158--165",
journal = "Biochimica et Biophysica Acta - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Protochlorophyll forms with different molecular arrangements.

AU - Böddi, B.

AU - Soós, J.

AU - Láng, F.

PY - 1980/11/5

Y1 - 1980/11/5

N2 - Spectral properties of protochlorophyll ()PChl) forms were investigated in solid-film model systems by absorption fluorescence and circular dichroism (CD) spectroscopy. The solid films were prepared from diethyl ether solution of PChl on a cover glass surface by evaporation of the solvent. After preparation the films usually showed an absoprtion maximum at 635 nm or in some cases at 640 nm. The PChl form with 635 nm absorption maximum had no CD signal, whilst the films with absorption maximum at 640 nm gave an intense negative CD band at about 640 nm and a positive one at 668 nm. The treatment of the films with ammonia or acetone vapour resulted in a red shift of the absorption maximum from 635 nm or 640 nm to 650 nm. The study of the CD spectra of the films with different PChl forms showed that, depending on the treatment, forms of PChl with similar absorption and fluorescence spectra, but with opposite CD signals, can exist. It is suggested that the differences of the CD spectra are mainly due to different arrangements of the aggregates.

AB - Spectral properties of protochlorophyll ()PChl) forms were investigated in solid-film model systems by absorption fluorescence and circular dichroism (CD) spectroscopy. The solid films were prepared from diethyl ether solution of PChl on a cover glass surface by evaporation of the solvent. After preparation the films usually showed an absoprtion maximum at 635 nm or in some cases at 640 nm. The PChl form with 635 nm absorption maximum had no CD signal, whilst the films with absorption maximum at 640 nm gave an intense negative CD band at about 640 nm and a positive one at 668 nm. The treatment of the films with ammonia or acetone vapour resulted in a red shift of the absorption maximum from 635 nm or 640 nm to 650 nm. The study of the CD spectra of the films with different PChl forms showed that, depending on the treatment, forms of PChl with similar absorption and fluorescence spectra, but with opposite CD signals, can exist. It is suggested that the differences of the CD spectra are mainly due to different arrangements of the aggregates.

UR - http://www.scopus.com/inward/record.url?scp=0019317721&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019317721&partnerID=8YFLogxK

M3 - Article

C2 - 7426643

AN - SCOPUS:0019317721

VL - 593

SP - 158

EP - 165

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

SN - 0304-4165

IS - 1

ER -