Protochlorophyll forms in roots of dark‐grown plants

Birgitta McEwen, Hemming I. Virgin, B. Böddi, Christer Sundqvist

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Protochlorophyll was found in roots of dark‐grown plants of seven species investigated. It was identified by absorbance and fluorescence spectra of acetone and ether extracts. Chlorophyll was also found in roots of one pea species. The concentration of protochlorophyll was usually highest in young root tips and decreased upwards along the roots. The maxima of the in vivo absorbance spectra of the species studied varied between 634 and 638 nm. Low temperature in vivo fluorescence emission spectra had two maxima, one at ca 633 and the other at ca 642 nm, when the wavelengths of the excitation light were 440 and 460 nm, respectively. In vivo fluorescence excitation spectra displayed a shift of the excitation maximum from 438 to 445 nm, when emission varied from 620 to 647.5 nm. Deconvolution of these three types of spectra into Gaussian components made it possible to identify two spectral forms of protochlorophyll: protochlorophyll629–633 and protochlorophyll638–642.

Original languageEnglish
Pages (from-to)455-461
Number of pages7
JournalPhysiologia Plantarum
Volume81
Issue number4
DOIs
Publication statusPublished - 1991

Fingerprint

protochlorophyll
Plant Roots
Fluorescence
fluorescence
absorbance
Meristem
Peas
Chlorophyll
Acetone
root tips
Ether
acetone
wavelengths
ethers
peas
chlorophyll
Light
Temperature
extracts
temperature

Keywords

  • Absorption spectra
  • chlorophyll
  • fluorescence spectra
  • Gaussian components
  • protochlorophyll
  • roots

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Protochlorophyll forms in roots of dark‐grown plants. / McEwen, Birgitta; Virgin, Hemming I.; Böddi, B.; Sundqvist, Christer.

In: Physiologia Plantarum, Vol. 81, No. 4, 1991, p. 455-461.

Research output: Contribution to journalArticle

McEwen, Birgitta ; Virgin, Hemming I. ; Böddi, B. ; Sundqvist, Christer. / Protochlorophyll forms in roots of dark‐grown plants. In: Physiologia Plantarum. 1991 ; Vol. 81, No. 4. pp. 455-461.
@article{2d0ebbd0acb740aaab30e7b31ae4b446,
title = "Protochlorophyll forms in roots of dark‐grown plants",
abstract = "Protochlorophyll was found in roots of dark‐grown plants of seven species investigated. It was identified by absorbance and fluorescence spectra of acetone and ether extracts. Chlorophyll was also found in roots of one pea species. The concentration of protochlorophyll was usually highest in young root tips and decreased upwards along the roots. The maxima of the in vivo absorbance spectra of the species studied varied between 634 and 638 nm. Low temperature in vivo fluorescence emission spectra had two maxima, one at ca 633 and the other at ca 642 nm, when the wavelengths of the excitation light were 440 and 460 nm, respectively. In vivo fluorescence excitation spectra displayed a shift of the excitation maximum from 438 to 445 nm, when emission varied from 620 to 647.5 nm. Deconvolution of these three types of spectra into Gaussian components made it possible to identify two spectral forms of protochlorophyll: protochlorophyll629–633 and protochlorophyll638–642.",
keywords = "Absorption spectra, chlorophyll, fluorescence spectra, Gaussian components, protochlorophyll, roots",
author = "Birgitta McEwen and Virgin, {Hemming I.} and B. B{\"o}ddi and Christer Sundqvist",
year = "1991",
doi = "10.1111/j.1399-3054.1991.tb05084.x",
language = "English",
volume = "81",
pages = "455--461",
journal = "Physiologia Plantarum",
issn = "0031-9317",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Protochlorophyll forms in roots of dark‐grown plants

AU - McEwen, Birgitta

AU - Virgin, Hemming I.

AU - Böddi, B.

AU - Sundqvist, Christer

PY - 1991

Y1 - 1991

N2 - Protochlorophyll was found in roots of dark‐grown plants of seven species investigated. It was identified by absorbance and fluorescence spectra of acetone and ether extracts. Chlorophyll was also found in roots of one pea species. The concentration of protochlorophyll was usually highest in young root tips and decreased upwards along the roots. The maxima of the in vivo absorbance spectra of the species studied varied between 634 and 638 nm. Low temperature in vivo fluorescence emission spectra had two maxima, one at ca 633 and the other at ca 642 nm, when the wavelengths of the excitation light were 440 and 460 nm, respectively. In vivo fluorescence excitation spectra displayed a shift of the excitation maximum from 438 to 445 nm, when emission varied from 620 to 647.5 nm. Deconvolution of these three types of spectra into Gaussian components made it possible to identify two spectral forms of protochlorophyll: protochlorophyll629–633 and protochlorophyll638–642.

AB - Protochlorophyll was found in roots of dark‐grown plants of seven species investigated. It was identified by absorbance and fluorescence spectra of acetone and ether extracts. Chlorophyll was also found in roots of one pea species. The concentration of protochlorophyll was usually highest in young root tips and decreased upwards along the roots. The maxima of the in vivo absorbance spectra of the species studied varied between 634 and 638 nm. Low temperature in vivo fluorescence emission spectra had two maxima, one at ca 633 and the other at ca 642 nm, when the wavelengths of the excitation light were 440 and 460 nm, respectively. In vivo fluorescence excitation spectra displayed a shift of the excitation maximum from 438 to 445 nm, when emission varied from 620 to 647.5 nm. Deconvolution of these three types of spectra into Gaussian components made it possible to identify two spectral forms of protochlorophyll: protochlorophyll629–633 and protochlorophyll638–642.

KW - Absorption spectra

KW - chlorophyll

KW - fluorescence spectra

KW - Gaussian components

KW - protochlorophyll

KW - roots

UR - http://www.scopus.com/inward/record.url?scp=84989730029&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84989730029&partnerID=8YFLogxK

U2 - 10.1111/j.1399-3054.1991.tb05084.x

DO - 10.1111/j.1399-3054.1991.tb05084.x

M3 - Article

AN - SCOPUS:84989730029

VL - 81

SP - 455

EP - 461

JO - Physiologia Plantarum

JF - Physiologia Plantarum

SN - 0031-9317

IS - 4

ER -