Abstract
The steady-state and time-resolved fluorescence characteristics of protochlorophyll (Pchl) dissolved in neat Triton X-100 and in Triton X-100 micelles were investigated, and the fluorescence lifetimes of different Pchl spectral forms were studied. Varying the concentration of Pchl or diluting the micellar solutions either with a buffer or with a micellar solution, 631-634, 645-655, 680-692 and above 700 nm emitting Pchl complexes were prepared, the ratios of which varied from one another. The fluorescence decay of the 631-634 nm emitting (monomeric) form had a mono-exponential character with a 5.4-ns fluorescence lifetime. The long-wavelength Pchl complexes (aggregates) had two fluorescence lifetime values within a range of 1.4-3.9 ns and 0.15-0.84 ns, which showed high variability in different environments. Depending on the conditions, either mono- or double-exponential fluorescence decay was found for a fluorescence band at 680-685 nm. These data show that despite their very similar steady-state fluorescence properties, Pchl complexes can differ in fluorescence lifetimes, which may reflect different molecular structures, intrinsic geometries or different molecular interactions. This underlines the importance of complex spectroscopic analysis for a precise description of native and artificial chlorophyllous pigment forms.
| Original language | English |
|---|---|
| Pages (from-to) | 262-271 |
| Number of pages | 10 |
| Journal | Journal of Photochemistry and Photobiology, B: Biology |
| Volume | 86 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 1 2007 |
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Keywords
- Fluorescence emission spectra
- Fluorescence lifetime
- Micelles
- Protochlorophyll
- Protochlorophyll aggregation
- Triton X-100
ASJC Scopus subject areas
- Plant Science
- Bioengineering
- Physical and Theoretical Chemistry
Cite this
Protochlorophyll complexes with similar steady-state fluorescence characteristics can differ in fluorescence lifetimes. A model study in Triton X-100. / Myśliwa-Kurdziel, Beata; Solymosi, K.; Kruk, Jerzy; Böddi, B.; Strzałka, Kazimierz.
In: Journal of Photochemistry and Photobiology, B: Biology, Vol. 86, No. 3, 01.03.2007, p. 262-271.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protochlorophyll complexes with similar steady-state fluorescence characteristics can differ in fluorescence lifetimes. A model study in Triton X-100
AU - Myśliwa-Kurdziel, Beata
AU - Solymosi, K.
AU - Kruk, Jerzy
AU - Böddi, B.
AU - Strzałka, Kazimierz
PY - 2007/3/1
Y1 - 2007/3/1
N2 - The steady-state and time-resolved fluorescence characteristics of protochlorophyll (Pchl) dissolved in neat Triton X-100 and in Triton X-100 micelles were investigated, and the fluorescence lifetimes of different Pchl spectral forms were studied. Varying the concentration of Pchl or diluting the micellar solutions either with a buffer or with a micellar solution, 631-634, 645-655, 680-692 and above 700 nm emitting Pchl complexes were prepared, the ratios of which varied from one another. The fluorescence decay of the 631-634 nm emitting (monomeric) form had a mono-exponential character with a 5.4-ns fluorescence lifetime. The long-wavelength Pchl complexes (aggregates) had two fluorescence lifetime values within a range of 1.4-3.9 ns and 0.15-0.84 ns, which showed high variability in different environments. Depending on the conditions, either mono- or double-exponential fluorescence decay was found for a fluorescence band at 680-685 nm. These data show that despite their very similar steady-state fluorescence properties, Pchl complexes can differ in fluorescence lifetimes, which may reflect different molecular structures, intrinsic geometries or different molecular interactions. This underlines the importance of complex spectroscopic analysis for a precise description of native and artificial chlorophyllous pigment forms.
AB - The steady-state and time-resolved fluorescence characteristics of protochlorophyll (Pchl) dissolved in neat Triton X-100 and in Triton X-100 micelles were investigated, and the fluorescence lifetimes of different Pchl spectral forms were studied. Varying the concentration of Pchl or diluting the micellar solutions either with a buffer or with a micellar solution, 631-634, 645-655, 680-692 and above 700 nm emitting Pchl complexes were prepared, the ratios of which varied from one another. The fluorescence decay of the 631-634 nm emitting (monomeric) form had a mono-exponential character with a 5.4-ns fluorescence lifetime. The long-wavelength Pchl complexes (aggregates) had two fluorescence lifetime values within a range of 1.4-3.9 ns and 0.15-0.84 ns, which showed high variability in different environments. Depending on the conditions, either mono- or double-exponential fluorescence decay was found for a fluorescence band at 680-685 nm. These data show that despite their very similar steady-state fluorescence properties, Pchl complexes can differ in fluorescence lifetimes, which may reflect different molecular structures, intrinsic geometries or different molecular interactions. This underlines the importance of complex spectroscopic analysis for a precise description of native and artificial chlorophyllous pigment forms.
KW - Fluorescence emission spectra
KW - Fluorescence lifetime
KW - Micelles
KW - Protochlorophyll
KW - Protochlorophyll aggregation
KW - Triton X-100
UR - http://www.scopus.com/inward/record.url?scp=33846603828&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846603828&partnerID=8YFLogxK
U2 - 10.1016/j.jphotobiol.2006.11.003
DO - 10.1016/j.jphotobiol.2006.11.003
M3 - Article
C2 - 17234426
AN - SCOPUS:33846603828
VL - 86
SP - 262
EP - 271
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
SN - 1011-1344
IS - 3
ER -