Proteomic study of the toxic effect of oligomeric Aβ1-42 in situ prepared from 'iso-Aβ1-42'

István Földi, Zsolt L. Datki, Zoltán Szabõ, Zsolt Bozsõ, Botond Penke, Tamás Janáky

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Alzheimer's disease (AD) is the most prevalent form of neurodegenerative disorders even so the exact pathomechanism is still unclear. Recently, it is widely accepted that amyloid-beta peptide (Aβ) toxicity is positively linked to Aβ oligomers, which may be responsible for the initiation of AD. For this reason, AD research requires well defined aggregation state and structure of Aβ. Precursor peptide 'iso-Aβ1-42' makes it possible to use Aβ1-42 with well- defined aggregation state for in vitro and in vivo experiments. The aim of this study was to identify protein expression changes from differentiated SH-SY5Y neuroblastoma cells after treatment with oligomeric Aβ1-42 prepared in situ from 'iso-Aβ1-42'. In our experiment, a cell viability assay revealed a strong and time-dependent toxic effect of oligomeric Aβ1-42 which was supported by dramatic morphological changes. Our proteomics study also revealed numerous significant protein expression changes (22 proteins down- and 25 proteins up-regulated) after comparison of the untreated and Aβ1-42-treated cell lysates by two-dimensional electrophoresis. From the functional classification of the identified proteins, we found deregulations of proteins involved in metabolic processes, cytoskeleton organisation and protein biosynthesis and a huge number of up-regulated stress proteins displayed oligomeric Aβ1-42-induced cell stress.

Original languageEnglish
Pages (from-to)691-702
Number of pages12
JournalJournal of Neurochemistry
Volume117
Issue number4
DOIs
Publication statusPublished - May 2011

Fingerprint

Poisons
Proteomics
Alzheimer Disease
Proteins
Agglomeration
Cells
Amyloid beta-Peptides
Protein Biosynthesis
Heat-Shock Proteins
Deregulation
Cytoskeleton
Neuroblastoma
Biosynthesis
Neurodegenerative Diseases
Electrophoresis
Oligomers
Cell Survival
Toxicity
Assays
Experiments

Keywords

  • Alzheimer's disease
  • iso-Aβ1-42
  • proteomics
  • SH-SY5Y cell line
  • two-dimensional electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Proteomic study of the toxic effect of oligomeric Aβ1-42 in situ prepared from 'iso-Aβ1-42'. / Földi, István; Datki, Zsolt L.; Szabõ, Zoltán; Bozsõ, Zsolt; Penke, Botond; Janáky, Tamás.

In: Journal of Neurochemistry, Vol. 117, No. 4, 05.2011, p. 691-702.

Research output: Contribution to journalArticle

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