Proteolytic activation of protein kinase C in the extracts of cells treated for a short time with phorbol ester

László Buday, János Seprödi, Gyöngyi Farkas, György Mészáros, Tibor Romhányi, Gábor Bánhegyi, József Mandl, Ferenc Antoni, Anna Faragó

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Abstract

A 10 min treatment of human neutrophils with phorbol 12-myristate 13-acetate (PMA) has been reported to induce accumulation of the proteolytically activated Ca2 +/phospholipid-independent catalytic fragment of protein kinase C in the cytosol of intact cells [(1986) J. Biol. Chem. 261, 4101-4105]. We investigated the proteolytic conversion of protein kinase C to the Ca2 +/phospholipid-independent form in the cytosol and membrane fractions of pig neutrophils. The activity of protein kinase C was measured with its specific oligopeptide substrate Ala-Ala-Ala-Ser-Phe-Lys-Ala-Lys-Lys-amide designed previously. In our experiments the short-term treatment of neutrophils with PMA did not induce the accumulation of the proteolytically activated form of protein kinase C in the cytosol of intact cells. However, treatment of cells with PMA enhanced the limited proteolysis of protein kinase C during the preparation of cell extracts.

Original languageEnglish
Pages (from-to)15-19
Number of pages5
JournalFEBS Letters
Volume223
Issue number1
DOIs
Publication statusPublished - Oct 19 1987

Fingerprint

Phorbol Esters
Cell Extracts
Protein Kinase C
Chemical activation
Cytosol
Acetates
Neutrophils
Phospholipids
Proteolysis
Oligopeptides
Amides
Swine
Cells
Membranes
Substrates
phorbol-12-myristate
Experiments

Keywords

  • (Neutrophil, Lymphocyte, Hepatocyte)
  • Phorbol ester
  • Protein kinase C
  • Proteolytic activation
  • Synthetic peptide substrate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Proteolytic activation of protein kinase C in the extracts of cells treated for a short time with phorbol ester. / Buday, László; Seprödi, János; Farkas, Gyöngyi; Mészáros, György; Romhányi, Tibor; Bánhegyi, Gábor; Mandl, József; Antoni, Ferenc; Faragó, Anna.

In: FEBS Letters, Vol. 223, No. 1, 19.10.1987, p. 15-19.

Research output: Contribution to journalArticle

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