Protein synthesis blockade differentially affects the stress-induced transcriptional activation of neuropeptide genes in parvocellular neurosecretory neurons

K. Kovács, Carlos Arias, Paul E. Sawchenko

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Corticotropin-releasing factor (CRF) and arginine vasopressin (AVP) are synergistically interacting ACTH secretagogues that are co-expressed by parvocellular neurosecretory neurons of the hypothalamic paraventricular nucleus (PVH). To shed light on the mechanisms that mediate the stress-induced transcriptional activation of these neuropeptide genes, quantitative hybridization histochemical methods were used to assess the effects of systemic treatment with the protein synthesis inhibitor, cycloheximide, on the ether stress-induced upregulation of primary CRF and AVP transcripts, in vivo. Pretreatment with cycloheximide prevented the induction of Fos, but not CREB phosphorylation, normally seen in response to acute ether exposure, and significantly attenuated the stress-induced rise in AVP, but not CRF, heteronuclear RNA expression in the parvocellular division of the PVH. These results support the view that distinct molecular mechanisms govern the expression of the two principal corticotropin-releasing factors, in vivo.

Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalMolecular Brain Research
Volume54
Issue number1
DOIs
Publication statusPublished - Feb 1998

Fingerprint

Corticotropin-Releasing Hormone
Neuropeptides
Transcriptional Activation
Arginine Vasopressin
Neurons
Cycloheximide
Ether
Genes
Proteins
Protein Synthesis Inhibitors
Paraventricular Hypothalamic Nucleus
Adrenocorticotropic Hormone
Up-Regulation
Phosphorylation
RNA

Keywords

  • c-fos
  • Corticotropin-releasing factor
  • Cycloheximide
  • Hypothalamus
  • Paraventricular nucleus
  • Transcription factors
  • Vasopressin

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Protein synthesis blockade differentially affects the stress-induced transcriptional activation of neuropeptide genes in parvocellular neurosecretory neurons. / Kovács, K.; Arias, Carlos; Sawchenko, Paul E.

In: Molecular Brain Research, Vol. 54, No. 1, 02.1998, p. 85-91.

Research output: Contribution to journalArticle

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