Protein structure is changed in psoriatic skin on the unaffected region - Imaging possibility with ATR-FTIR spectroscopy

Boglárka Balázs, Gabriella Farkas, O. Berkesi, R. Gyulai, Szilvia Berkó, Mária Budai-Szucs, P. Szabó-Révész, L. Kemény, Erzsébet Csányi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Psoriasis is a T lymphocyte-mediated inflammatory disorder that affects the skin. A number of studies have demonstrated the occurrence of lipid alterations in psoriatic skin, resulting in a highly perturbed stratum corneum (SC). Relatively little attention has been paid to the protein conformation of the SC. In this study, the attenuated total reflection Fourier transform infrared (ATR-FTIR) spectrum of the untreated psoriatic patients' unharmed SC was obtained after tape stripping. We focused on the amide-I band components in order to establish whether there are any protein alterations in the intact areas of psoriatic skin. Fourier self-deconvolution (FSD) of the amide-I band was followed by curve-fitting to generate the underlying components. Integration of band areas provided an estimate of the secondary structure. The results indicated decreases in all amide-I band components, the peak at 1660cm-1 revealing the most dramatic change. This peak is characteristic of the turn structure in the protein chain. The decrease is marked in the case of the β-sheet structure at 1630cm-1 too. This ATR-FTIR imaging is a rapid and simple noninvasive method, promotes a better understanding of the disease, and would be helpful in following the treatment.

Original languageEnglish
Pages (from-to)183-186
Number of pages4
JournalMicrochemical Journal
Volume117
DOIs
Publication statusPublished - 2014

Fingerprint

Amides
Skin
Imaging techniques
Fourier transforms
Proteins
T-cells
Infrared imaging
Curve fitting
Deconvolution
Tapes
Conformations
Infrared radiation
Lipids

Keywords

  • Amide-I band
  • ATR-FTIR spectroscopy
  • Fourier self-deconvolution
  • Protein structure
  • Psoriatic skin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Protein structure is changed in psoriatic skin on the unaffected region - Imaging possibility with ATR-FTIR spectroscopy. / Balázs, Boglárka; Farkas, Gabriella; Berkesi, O.; Gyulai, R.; Berkó, Szilvia; Budai-Szucs, Mária; Szabó-Révész, P.; Kemény, L.; Csányi, Erzsébet.

In: Microchemical Journal, Vol. 117, 2014, p. 183-186.

Research output: Contribution to journalArticle

Balázs, Boglárka ; Farkas, Gabriella ; Berkesi, O. ; Gyulai, R. ; Berkó, Szilvia ; Budai-Szucs, Mária ; Szabó-Révész, P. ; Kemény, L. ; Csányi, Erzsébet. / Protein structure is changed in psoriatic skin on the unaffected region - Imaging possibility with ATR-FTIR spectroscopy. In: Microchemical Journal. 2014 ; Vol. 117. pp. 183-186.
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