Emerging proteomic techniques offer new approaches for identifying tumor biomarkers. Surface enhanced laser desorption/ionization mass spectrometry (SELDIMS) is a novel method for biomarker discovery that can provide a rapid protein expression profile from a variety of biological samples. Since SELDI-MS method requires a small amount of biological material, this technique is ideal for analyzing proteins procured by Laser Capture Microdissection (LCM). Current study was undertaken to investigate potential differences in protein expression between normal placenta and complete mole utilizing SELDI-MS ProteinChip technology on microdissected trophoblast cells. Nine samples from complete hydatidiform moles and ten from normal placentas of comparable gestational age were analyzed. Four metal binding polypeptides were identified between 2.8 and 20 kDa molecular weight. Three of them were found at significantly higher intensity in normal placentas than in complete moles. While further characterization of these protein peaks in normal and molar pregnancy is important and necessary, our current work demonstrates that combined technology of SELDI-MS and LCM is effective in protein expression analysis of normal placenta and complete mole. Hopefully additional investigations may advance our knowledge of protein expression in mole, which may improve our understanding in the pathogenesis and molecular mechanisms of gestational trophoblastic diseases.
|Number of pages||6|
|Journal||Magyar Noorvosok Lapja|
|Publication status||Published - Jun 30 2003|
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynaecology