Protein profile characterization of bacterial lysates by capillary electrophoresis

Ildikó Kustos, Béla Kocsis, Ildikó Kerepesi, Ferenc Kilár

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A fast and reproducible method was developed to characterize cell lysates by their electrophoretic profiles using capillary electrophoresis (CE). Characteristic and reproducible patterns were recorded for each bacterial strains when 'dynamic sieving' CE, using a polymer solution in the capillary, was applied to distinguish four strains of the Enterobacteriaceae family. The electropherograms showed distinct differences when comparing them to the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protein profiles. This is certainly a result of the differences in the separation principles and in the detection methods of the two techniques.

Original languageEnglish
Pages (from-to)2317-2323
Number of pages7
JournalELECTROPHORESIS
Volume19
Issue number13
DOIs
Publication statusPublished - Oct 1 1998

Keywords

  • Bacteria
  • Capillary electrophoresis
  • Enterobacteriaceae family
  • Lysate
  • Protein profile

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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