Protein phosphatase Z modulates oxidative stress response in fungi

Éva Leiter, Asier González, Éva Erdei, Carlos Casado, László Kovács, Csaba ádám, Judit Oláh, Márton Miskei, Monika Molnar, Ilona Farkas, Zsuzsanna Hamari, Joaquín Ariño, István Pócsi, Viktor Dombrádi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The genome of the filamentous fungus Aspergillus nidulans harbors the gene ppzA that codes for the catalytic subunit of protein phosphatase Z (PPZ), and the closely related opportunistic pathogen Aspergillus fumigatus encompasses a highly similar PPZ gene (phzA). When PpzA and PhzA were expressed in Saccharomyces cerevisiae or Schizosaccharomyces pombe they partially complemented the deleted phosphatases in the ppz1 or the pzh1 mutants, and they also mimicked the effect of Ppz1 overexpression in slt2 MAP kinase deficient S. cerevisiae cells. Although ppzA acted as the functional equivalent of the known PPZ enzymes its disruption in A. nidulans did not result in the expected phenotypes since it failed to affect salt tolerance or cell wall integrity. However, the inactivation of ppzA resulted in increased sensitivity to oxidizing agents like tert-butylhydroperoxide, menadione, and diamide. To demonstrate the general validity of our observations we showed that the deletion of the orthologous PPZ genes in other model organisms, such as S. cerevisiae (PPZ1) or Candida albicans (CaPPZ1) also caused oxidative stress sensitivity. Thus, our work reveals a novel function of the PPZ enzyme in A. nidulans that is conserved in very distantly related fungi.

Original languageEnglish
Pages (from-to)708-716
Number of pages9
JournalFungal Genetics and Biology
Volume49
Issue number9
DOIs
Publication statusPublished - Sep 1 2012

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Keywords

  • Aspergillus (Emericella) nidulans
  • Diamide
  • Menadione
  • Oxidative stress
  • Protein phosphatase Z
  • Tert-Butylhydroperoxide

ASJC Scopus subject areas

  • Microbiology
  • Genetics

Cite this

Leiter, É., González, A., Erdei, É., Casado, C., Kovács, L., ádám, C., Oláh, J., Miskei, M., Molnar, M., Farkas, I., Hamari, Z., Ariño, J., Pócsi, I., & Dombrádi, V. (2012). Protein phosphatase Z modulates oxidative stress response in fungi. Fungal Genetics and Biology, 49(9), 708-716. https://doi.org/10.1016/j.fgb.2012.06.010