Protein phosphatase 2A is involved in hyphal growth of Neurospora crassa

E. Yatzkan, B. Szöor, Z. Fehér, V. Dombrádi, O. Yarden

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Cantharidin and calyculin A, natural toxins that are inhibitors of protein phosphatases 1 and 2A (PP1 and PP2A, respectively), inhibit Neurospora crassa hyphal growth. When N. cassa was grown in the presence of either drug, abnormalities were observed at hyphal tips. In addition, both drugs induced an increase in hyphal branching. Cantharidin inhibited N. crassa hyphal growth in a temperature-dependent manner, as the effect of the drug was more pronounced at 34°C than at 25°C. In addition to the drug-mediated inhibition of phosphatase activity, a genetic approach was used to determine the phenotypic consequences of reduced PP2A activity. Two strains with subnormal PP2A activity were constructed. The first, in which the original pph-1 gene (encoding the PP2A catalytic subunit) was replaced with an ectopically integrated copy of pph-1, exhibited lower levels of pph-1 transcript, lower PP2A activity and increased sensitivity to cantharidin. Similarly, in a second strain, in which the pph-1 gene was cloned in an antisense orientation downstream of the inducible isocitrate lyase promoter, lower levels of pph-1 transcript, as well as of PP2A activity, and a reduction in hyphal growth were observed. The results of this study indicate that PP2A, and probably other Ser/Thr phosphatases, are involved in the regulation of hyphal growth in N. crassa.

Original languageEnglish
Pages (from-to)523-531
Number of pages9
JournalMGG Molecular & General Genetics
Volume259
Issue number5
DOIs
Publication statusPublished - 1998

Fingerprint

Protein Phosphatase 2
Neurospora crassa
Cantharidin
Growth
Phosphoric Monoester Hydrolases
Pharmaceutical Preparations
Isocitrate Lyase
Genes
Catalytic Domain
Temperature

Keywords

  • Calyculin A
  • Cantharidin
  • Neurospora crassa
  • Okadaic acid
  • Protein phosphatase 2A

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Protein phosphatase 2A is involved in hyphal growth of Neurospora crassa. / Yatzkan, E.; Szöor, B.; Fehér, Z.; Dombrádi, V.; Yarden, O.

In: MGG Molecular & General Genetics, Vol. 259, No. 5, 1998, p. 523-531.

Research output: Contribution to journalArticle

Yatzkan, E. ; Szöor, B. ; Fehér, Z. ; Dombrádi, V. ; Yarden, O. / Protein phosphatase 2A is involved in hyphal growth of Neurospora crassa. In: MGG Molecular & General Genetics. 1998 ; Vol. 259, No. 5. pp. 523-531.
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