Protein phosphatase 1 activity in Drosophila mutants with abnormalities in mitosis and chromosome condensation

V. Dombrádi, J. Myles Axton, Hazel M. Barker, Patricia T W Cohen

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

A Drosophila gene encoding a protein phosphatase 1(PPI) has been sequenced, and lethal mutations in this locus (87B) analysed. Two mutants (ck19e211 and ck19hs46), which disrupt mitosis, lack the 87B isoenzyme and express only ∼20% of wild type PPI activity. The Promoter region of the gene is deleted in the ck19e11 mutant. A third mutant (ck19e078), which shows suppression of position effect variegation, but has little effect on mitosis, possesses ∼35% of wild type PPI activity. The results indicate that the PPI 87B isoenzyme is involved in regulation of chromosome condensation at interphase as well as mitosis.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalFEBS Letters
Volume275
Issue number1-2
DOIs
Publication statusPublished - Nov 26 1990

Fingerprint

Protein Phosphatase 1
Chromosomes
Mitosis
Isoenzymes
Drosophila
Condensation
Gene encoding
Chromosomal Position Effects
Genetic Promoter Regions
Genes
Interphase
Mutation

Keywords

  • Drosophila
  • Gene sequence
  • Mitosis
  • Position effect variegation
  • Protein phosphatase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Protein phosphatase 1 activity in Drosophila mutants with abnormalities in mitosis and chromosome condensation. / Dombrádi, V.; Axton, J. Myles; Barker, Hazel M.; Cohen, Patricia T W.

In: FEBS Letters, Vol. 275, No. 1-2, 26.11.1990, p. 39-43.

Research output: Contribution to journalArticle

Dombrádi, V. ; Axton, J. Myles ; Barker, Hazel M. ; Cohen, Patricia T W. / Protein phosphatase 1 activity in Drosophila mutants with abnormalities in mitosis and chromosome condensation. In: FEBS Letters. 1990 ; Vol. 275, No. 1-2. pp. 39-43.
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