Protein kinase D controls the integrity of golgi apparatus and the maintenance of dendritic arborization in hippocampal neurons

Katalin Czöndör, Kornelia Ellwanger, Yannick F. Fuchs, Sylke Lutz, Márton Gulyás, Isabelle M. Mansuy, Angelika Hausser, Klaus Pfizenmaier, Katalin Schlett

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33 Citations (Scopus)

Abstract

Protein kinase D (PKD) is known to participate in various cellular functions, including secretory vesicle fission from the Golgi and plasma membrane-directed transport. Here, we report on expression and function of PKD in hippocampal neurons. Expression of an enhanced green fluorescent protein (EGFP)-tagged PKD activity reporter in mouse embryonal hippocampal neurons revealed high endogenous PKD activity at the Golgi complex and in the dendrites, whereas PKD activity was excluded from the axon in parallel with axonal maturation. Expression of fluorescently tagged wild-type PKD1 and constitutively active PKD1S738/742E (caPKDl) in neurons revealed that both proteins were slightly enriched at the trans-Golgi network (TGN) and did not interfere with its thread-like morphology. By contrast, expression of dominant-negative kinase inactive PKD1K612W (kdPKDl) led to the disruption of the neuronal Golgi complex, with kdPKDl strongly localized to the TGN fragments. Similar findings were obtained from transgenic mice with inducible, neuron-specific expression of kdPKDl-EGFP. As a prominent consequence of kdPKDl expression, the dendritic tree of transfected neurons was reduced, whereas caPKDl increased dendritic arborization. Our results thus provide direct evidence that PKD activity is selectively involved in the maintenance of dendritic arborization and Golgi structure of hippocampal neurons.

Original languageEnglish
Pages (from-to)2108-2120
Number of pages13
JournalMolecular Biology of the Cell
Volume20
Issue number7
DOIs
Publication statusPublished - Apr 1 2009

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Czöndör, K., Ellwanger, K., Fuchs, Y. F., Lutz, S., Gulyás, M., Mansuy, I. M., Hausser, A., Pfizenmaier, K., & Schlett, K. (2009). Protein kinase D controls the integrity of golgi apparatus and the maintenance of dendritic arborization in hippocampal neurons. Molecular Biology of the Cell, 20(7), 2108-2120. https://doi.org/10.1091/mbc.E08-09-0957