Protein kinase C is involved in the early signals of interferon-α but not of interferon-γ in U937 cells

P. Csermely, E. Balint, P. M. Grimley, A. Aszalos

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Increasing interest is being focused on the role of protein kinase C (PKC) in the mode of action of interferons (IFNs). Here we report that IFN-α induced a transient translocation of PKC from the cytosol to the particulate fraction of U937 cells. In contrast, after IFN-γ treatment, no significant change in PKC activity could be observed. IFN-induced changes in membrane potential were also examined by means of a potential sensitive oxonol dye and flow cytometry. Hyperpolarization was induced by both IFN-α and IFN-γ. The protein kinase inhibitor H-7 blocked the hyperpolarization induced by IFN-α but not by IFN-γ. These concordant results suggest that PKC is involved in the early signals of IFN-α but not of IFN-γ in U937 cells.

Original languageEnglish
Pages (from-to)605-611
Number of pages7
JournalJournal of Interferon Research
Volume10
Issue number6
Publication statusPublished - 1990

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U937 Cells
Protein Kinase C
Interferons
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Protein Kinase Inhibitors
Membrane Potentials
Cytosol
Flow Cytometry
Coloring Agents

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Protein kinase C is involved in the early signals of interferon-α but not of interferon-γ in U937 cells. / Csermely, P.; Balint, E.; Grimley, P. M.; Aszalos, A.

In: Journal of Interferon Research, Vol. 10, No. 6, 1990, p. 605-611.

Research output: Contribution to journalArticle

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