Protein kinase C activation in Aplysia neurons by phorbol diacetate: comparison of effects following extracellular or intracellular application.

A. Papp, M. R. Klee

Research output: Contribution to journalArticle


The effects of protein kinase C activation on electrophysiological phenomena of neurons in Aplysia californica ganglions were studied. The enzyme was activated by phorbol-12,13-diacetate applied either extracellularly by perfusion, or by intracellular pressure injection. In both forms of application, an increase in the potential upstroke speed and amplitude as well as a reduction of the depolarization evoked by extracellular acetylcholine application was found. A protein kinase C blocker, H-7, had opposite effects on the action potential. All observed actions of the phorbol ester were consistently faster in intracellular application than extracellular.

Original languageEnglish
Pages (from-to)147-157
Number of pages11
JournalActa physiologica Hungarica
Issue number2
Publication statusPublished - 1993


ASJC Scopus subject areas

  • Physiology (medical)

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