Protein kinase Cδ promotes proliferation and induces malignant transformation in skeletal muscle

Gabriella Czifra, Attila Szöllosi, Zsuzsanna Nagy, Miklós Boros, István Juhász, Andrea Kiss, Ferenc Erdodi, Tamás Szabó, Ilona Kovács, Miklós Török, László Kovács, Peter M. Blumberg, Tamás Bíró

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this paper, we investigated the isoform-specific roles of certain protein kinase C (PKC) isoforms in the regulation of skeletal muscle growth. Here, we provide the first intriguing functional evidence that nPKCδ (originally described as an inhibitor of proliferation in various cells types) is a key player in promoting both in vitro and in vivo skeletal muscle growth. Recombinant overexpression of a constitutively active nPKCδ in C2C12 myoblast increased proliferation and inhibited differentiation. Conversely, overexpression of kinase-negative mutant of nPKCδ (DN-nPKCδ) markedly inhibited cell growth. Moreover, overexpression of nPKCδ also stimulated in vivo tumour growth and induced malignant transformation in immunodeficient (SCID) mice whereas that of DN-nPKCδ suppressed tumour formation. The role of nPKCδ in the formation of rhabdomyosarcoma was also investigated where recombinant overexpression of nPKCδ in human rhabdomyosarcoma RD cells also increased cell proliferation and enhanced tumour formation in mouse xenografts. The other isoforms investigated (PKCα, β, ε) exerted only minor (mostly growth-inhibitory) effects in skeletal muscle cells. Collectively, our data introduce nPKCδ as a novel growth-promoting molecule in skeletal muscles and invite further trials to exploit its therapeutic potential in the treatment of skeletal muscle malignancies.

Original languageEnglish
Pages (from-to)396-407
Number of pages12
JournalJournal of Cellular and Molecular Medicine
Volume19
Issue number2
DOIs
Publication statusPublished - Feb 1 2015

Keywords

  • C2C12 myoblasts
  • Differentiation
  • PKC isoenzymes
  • Proliferation
  • Protein kinase C
  • Recombinant overexpression
  • Rhabdomyosarcoma
  • Skeletal muscle
  • Tumourigenesis
  • nPKCδ

ASJC Scopus subject areas

  • Molecular Medicine
  • Cell Biology

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