Protein kinase A signaling and calcium ions are major players in PAF mediated toxicity against Aspergillus niger

Ulrike Binder, Mojca Benčina, Ádám Fizil, G. Batta, Anil K. Chhillar, Florentine Marx

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Abstract The Penicillium chrysogenum antifungal protein PAF is toxic against potentially pathogenic Ascomycetes. We used the highly sensitive aequorin-expressing model Aspergillus niger to identify a defined change in cytoplasmic free Ca2+ dynamics in response to PAF. This Ca2+ signature depended on an intact positively charged lysine-rich PAF motif. By combining Ca2+ measurements in A. niger mutants with deregulated cAMP/protein kinase A (PKA) signaling, we proved the interconnection of Ca2+ perturbation and cAMP/PKA signaling in the mechanistic function of PAF. A deep understanding of the mode of action of PAF is an invaluable prerequisite for its future application as new antifungal drug.

Original languageEnglish
Article number37099
Pages (from-to)1266-1271
Number of pages6
JournalFEBS Letters
Volume589
Issue number11
DOIs
Publication statusPublished - May 8 2015

Fingerprint

Calcium Signaling
Aspergillus niger
Aspergillus
Cyclic AMP-Dependent Protein Kinases
Toxicity
Aequorin
Ions
Calcium
Ascomycota
Poisons
Lysine
Pharmaceutical Preparations
Proteins
Penicillium chrysogenum PAF protein

Keywords

  • Antifungal protein
  • Aspergillus niger
  • Calcium homeostasis
  • PAF
  • Protein kinase A signaling

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Protein kinase A signaling and calcium ions are major players in PAF mediated toxicity against Aspergillus niger. / Binder, Ulrike; Benčina, Mojca; Fizil, Ádám; Batta, G.; Chhillar, Anil K.; Marx, Florentine.

In: FEBS Letters, Vol. 589, No. 11, 37099, 08.05.2015, p. 1266-1271.

Research output: Contribution to journalArticle

Binder, Ulrike ; Benčina, Mojca ; Fizil, Ádám ; Batta, G. ; Chhillar, Anil K. ; Marx, Florentine. / Protein kinase A signaling and calcium ions are major players in PAF mediated toxicity against Aspergillus niger. In: FEBS Letters. 2015 ; Vol. 589, No. 11. pp. 1266-1271.
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