Protein-DNA interaction and CpG methylation at rep*/vIL-10p of latent epstein-barr virus genomes in lymphoid cell lines

H. H. Niller, D. Salamon, M. Takács, J. Uhlig, H. Wolf, J. Mináróvits

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The viral interleukin-10 promoter (vIL-10p), overlapping the rep* element in the Epstein-Barr virus (EBV) genome, is a promoter element active mostly in the late phase of the lytic cycle and immediately upon infection of B cells. rep* was, through transfection experiments with small plasmids, characterised as a cis element supporting oriP replicative function. In this study, in vivo protein binding and CpG methylation at rep*/vIL-10p were analysed in five cell lines that harbour strictly latent EBV genomes. Contrary to the invariably unmethylated dyad symmetry element (DS) of oriP, rep*/vIL-10p was highly methylated and showed only traces of protein binding in all examined cell lines. This result is in agreement with vIL-10p being an inactive promoter of EBV genomes, and makes it less likely that rep* functions as a replicative element of latent EBV genomes.

Original languageEnglish
Pages (from-to)1411-1419
Number of pages9
JournalBiological Chemistry
Volume382
Issue number10
DOIs
Publication statusPublished - 2001

Fingerprint

Methylation
Human Herpesvirus 4
Viruses
Interleukin-10
Genes
Cells
Genome
Lymphocytes
Cell Line
DNA
Protein Binding
Proteins
Ports and harbors
Transfection
Plasmids
B-Lymphocytes
Infection
Experiments

Keywords

  • Bisulfite sequencing
  • Dimethyl sulfate
  • Genomic footprints
  • Latency
  • Replication

ASJC Scopus subject areas

  • Biochemistry

Cite this

Protein-DNA interaction and CpG methylation at rep*/vIL-10p of latent epstein-barr virus genomes in lymphoid cell lines. / Niller, H. H.; Salamon, D.; Takács, M.; Uhlig, J.; Wolf, H.; Mináróvits, J.

In: Biological Chemistry, Vol. 382, No. 10, 2001, p. 1411-1419.

Research output: Contribution to journalArticle

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AU - Takács, M.

AU - Uhlig, J.

AU - Wolf, H.

AU - Mináróvits, J.

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