Protein biocargo of citrus fruit-derived vesicles reveals heterogeneous transport and extracellular vesicle populations

Gabriella Pocsfalvi, Lilla Turiák, Alfredo Ambrosone, Pasquale del Gaudio, Gina Puska, Immacolata Fiume, Teresa Silvestre, K. Vékey

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cell-derived vesicles are membrane-enclosed organelles that transport material inside and outside the cell. Plant-derived vesicles are receiving more and more attention due to their potential as nanovectors for the delivery of biologically active substances. Here, we studied the heterogeneity and protein biocargo in citrus fruit juice sac cell-derived vesicles populations. Micro- and nano-sized vesicle fractions were isolated from four citrus species, C. sinensis, C. limon, C. paradisi and C. aurantium, characterized using physicochemical methods and protein cargos were compared using label-free quantitative shotgun proteomics. In each sample approximately 600–800 proteins were identified. Orthologues of most of the top-ranking proteins have previously been reported in extracellular vesicles of mammalian origin. High expression levels of patellin-3-like, clathrin heavy chain, heat shock proteins, 14-3-3 protein, glyceraldehyde-3-phosphate dehydrogenase and fructose-bisphosphate aldolase 6 were measured in all samples while aquaporin was highly expressed only in the nanovesicle fractions. Bioinformatics revealed more than hundred protein orthologues potentially implicated in vesicular trafficking. In particular, the presence of CCV, COPI and COPII coat proteins indicates the presence of heterogeneous populations of intracellular transport vesicles. Moreover, a high number of different enzymes including hydrolases and oxidoreductases are ubiquities in citrus fruit sac cell-derived vesicles.

Original languageEnglish
Pages (from-to)111-121
Number of pages11
JournalJournal of Plant Physiology
Volume229
DOIs
Publication statusPublished - Oct 1 2018

Fingerprint

Transport Vesicles
Citrus
citrus fruits
14-3-3 Proteins
Population
Proteins
proteins
Clathrin Heavy Chains
Coat Protein Complex I
Aquaporins
Fructose-Bisphosphate Aldolase
Glyceraldehyde-3-Phosphate Dehydrogenases
cells
citrus juices
Firearms
Capsid Proteins
clathrin
Hydrolases
fructose-bisphosphate aldolase
Heat-Shock Proteins

Keywords

  • Bioinformatics
  • Citrus
  • Enzymes
  • Extracellular vesicles
  • Fruit juice sac cell
  • Hydrolases
  • Nanovectors
  • Oxidoreductases
  • Proteomics
  • Vesicles
  • Vesicular trafficking

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Protein biocargo of citrus fruit-derived vesicles reveals heterogeneous transport and extracellular vesicle populations. / Pocsfalvi, Gabriella; Turiák, Lilla; Ambrosone, Alfredo; del Gaudio, Pasquale; Puska, Gina; Fiume, Immacolata; Silvestre, Teresa; Vékey, K.

In: Journal of Plant Physiology, Vol. 229, 01.10.2018, p. 111-121.

Research output: Contribution to journalArticle

Pocsfalvi, Gabriella ; Turiák, Lilla ; Ambrosone, Alfredo ; del Gaudio, Pasquale ; Puska, Gina ; Fiume, Immacolata ; Silvestre, Teresa ; Vékey, K. / Protein biocargo of citrus fruit-derived vesicles reveals heterogeneous transport and extracellular vesicle populations. In: Journal of Plant Physiology. 2018 ; Vol. 229. pp. 111-121.
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