Protein-based all-optical sensor device

A. Dér, Sándor Valkai, Anna Mathesz, I. Andó, Elmar K. Wolff, P. Ormos

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The integrated optical Mach-Zehnder interferometer is a highly sensitive device, considered a powerful lab-on-a-chip tool for specific detection of various chemical and biochemical reactions. Despite its advantages, there is no commercially available biosensor based on this technique. The main reason is the inherent instability of the device due to slight changes of environmental parameters. In this paper we offer a solution to this problem that enables the optimal adjustment of the working point of the sensor prior to the measurement. The key feature is a control unit made of a thin film of the light-sensitive chromoprotein bacteriorhodopsin deposited on the reference arm of the interferometer. After showing the transfer characteristics of such a device, we demonstrate its applicability to sensing of specific protein-protein interactions. We expect our method to become a rapid and cost-efficient alternative of the commonly used measuring tools in protein research and medical diagnostics.

Original languageEnglish
Pages (from-to)26-29
Number of pages4
JournalSensors and Actuators, B: Chemical
Volume151
Issue number1
DOIs
Publication statusPublished - Nov 26 2010

Fingerprint

Optical sensors
optical measuring instruments
proteins
Proteins
Bacteriorhodopsins
Lab-on-a-chip
Mach-Zehnder interferometers
bioinstrumentation
Biosensors
Interferometers
chemical reactions
interferometers
adjusting
chips
costs
Thin films
sensors
Sensors
thin films
Costs

Keywords

  • Bacteriorhodopsin
  • Biosensor
  • Mach-Zehnder interferometer

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Protein-based all-optical sensor device. / Dér, A.; Valkai, Sándor; Mathesz, Anna; Andó, I.; Wolff, Elmar K.; Ormos, P.

In: Sensors and Actuators, B: Chemical, Vol. 151, No. 1, 26.11.2010, p. 26-29.

Research output: Contribution to journalArticle

Dér, A. ; Valkai, Sándor ; Mathesz, Anna ; Andó, I. ; Wolff, Elmar K. ; Ormos, P. / Protein-based all-optical sensor device. In: Sensors and Actuators, B: Chemical. 2010 ; Vol. 151, No. 1. pp. 26-29.
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