Protein adsorption on functionalized and ESCA-characterized polymer films studied by ellipsometry

Carl Gustaf Gölander, E. Kiss

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

The adsorption of bovine serum albumin (BSA), immunoglobulin G (IgG), fibrinogen, and poly-l-lysine (PLL) on polyvinylchloride (PVC), a copolymer of methacrylic acid/methacrylate (PMA) and surface-grafted polyethylene oxide (PEO) films, has been studied by means of ellipsometry. The films were spin-cast on silicon wafers covered with silicon oxide (SiOx). Under physiological conditions, most proteins, but not the polyelectrolyte PLL, adsorbed to a larger extent to the hydrophobic PVC than to the hydrophilic PMA or PEO surfaces. For PLL extensive adsorption to PMA occurred as a result of strong electrostatic interaction. A correlation was found between the amount of protein adsorbed as measured by ellipsometry, and the relative surface content of nitrogen as recorded using ESCA. None of the proteins adsorbed to a constant plateau level. Instead, a monotonically increasing degree of adsorption was found in the concentration range studied, 0-1000 g/m3. For the hydrophobic PVC, the isotherms were similar in shape to those theoretically obtained by I. Lundström (Prog. Colloid Polym. Sci. 70, 76-82 (1985)) upon assuming a dynamic adsorption process which takes into account several modes of adsorption characterized by different affinities to the surface. Generally, a low degree of protein adsorption, Γ <0.5 mg/m2, was observed for surfaces covered with PEO chains (mol wt 1900) which were covalently linked, by means of terminal CHO groups, to surface amino groups.

Original languageEnglish
Pages (from-to)240-253
Number of pages14
JournalJournal of Colloid and Interface Science
Volume121
Issue number1
DOIs
Publication statusPublished - 1988

Fingerprint

Ellipsometry
Polymer films
ellipsometry
proteins
Proteins
Adsorption
adsorption
lysine
polymers
Polyethylene oxides
Lysine
polyethylenes
fibrinogen
oxides
Methacrylates
Silicon oxides
Colloids
Bovine Serum Albumin
Coulomb interactions
Polyelectrolytes

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Protein adsorption on functionalized and ESCA-characterized polymer films studied by ellipsometry. / Gölander, Carl Gustaf; Kiss, E.

In: Journal of Colloid and Interface Science, Vol. 121, No. 1, 1988, p. 240-253.

Research output: Contribution to journalArticle

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