Protein adsorption on detonation nanodiamond/polymer composite layers

Lilyana D. Pramatarova, Todor A. Hikov, Natalia A. Krasteva, P. Petrik, Raina P. Dimitrova, Emilia V. Pecheva, Ekaterina I. Radeva, Elot Agocs, Ivaylo G. Tsvetanov, Radina P. Presker

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Composite layers of the detonation nanodiamond/polymer type possess a spatial organization of components with new structural features and physical properties, as well as complex functions due to the strong synergistic effects between the nanoparticles and polymer [1]. Composite layers were deposited by a plasma polymerization (PP) process of the detonation nanodiamond (DND) particles added to a hexamethyl disiloxan (HMDS) monomer [1]. The incorporation of silver ions in the polymer leads to the production of materials that are highly efficient against bacterial colonization and allows better cell adhesion and spreading. [2] For cell culture processes, fibronectin (FN) treatment is one of the commonly used approaches to enhance the cell adhesion on a surface [3]. As an integrated part of our search for improved materials for life science applications such as biomaterials and biosensors, the objective of the present study is to investigate the interaction of Ag-based composite surfaces with FN protein. Two types of composite layers, Ag-ND/PPHMDS and Ag-nano/PPHMDS were obtained by plasma polymerization of HMDS and nanoparticles of Ag and Ag-DND. The composite layers are representative of the different incorporation of the Ag in the polymer net. The structures studied, consisting of composite layers with adsorbed FN were optically characterized with Ellipsometry, Fourier Transform Infrared (FTIR) and Ultra Violet (UV) Spectroscopy as well as by stylus profiling (Talysurf). The kinetic study of the FN adsorption indicates that the process depends on the FN concentration and the exposure time as well as on the surface chemistry of the composites. Compared to the reference sample, all composite layers exhibit an indication of a stronger ability to initiate the intrinsic pathway of coagulation.

Original languageEnglish
Title of host publicationMaterials Research Society Symposium Proceedings
Pages51-56
Number of pages6
Volume1479
DOIs
Publication statusPublished - 2013
Event21st International Materials Research Congress, IMRC 2012 - Cancun, Mexico
Duration: Aug 12 2012Aug 17 2012

Other

Other21st International Materials Research Congress, IMRC 2012
CountryMexico
CityCancun
Period8/12/128/17/12

Fingerprint

Nanodiamonds
Detonation
detonation
Polymers
proteins
Proteins
Adsorption
Fibronectins
adsorption
composite materials
Composite materials
polymers
Plasma polymerization
Cell adhesion
adhesion
polymerization
Nanoparticles
nanoparticles
life sciences
ultraviolet spectroscopy

Keywords

  • Adsorption
  • Nanostructures
  • Protein

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Pramatarova, L. D., Hikov, T. A., Krasteva, N. A., Petrik, P., Dimitrova, R. P., Pecheva, E. V., ... Presker, R. P. (2013). Protein adsorption on detonation nanodiamond/polymer composite layers. In Materials Research Society Symposium Proceedings (Vol. 1479, pp. 51-56) https://doi.org/10.1557/opl.2012.1597

Protein adsorption on detonation nanodiamond/polymer composite layers. / Pramatarova, Lilyana D.; Hikov, Todor A.; Krasteva, Natalia A.; Petrik, P.; Dimitrova, Raina P.; Pecheva, Emilia V.; Radeva, Ekaterina I.; Agocs, Elot; Tsvetanov, Ivaylo G.; Presker, Radina P.

Materials Research Society Symposium Proceedings. Vol. 1479 2013. p. 51-56.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Pramatarova, LD, Hikov, TA, Krasteva, NA, Petrik, P, Dimitrova, RP, Pecheva, EV, Radeva, EI, Agocs, E, Tsvetanov, IG & Presker, RP 2013, Protein adsorption on detonation nanodiamond/polymer composite layers. in Materials Research Society Symposium Proceedings. vol. 1479, pp. 51-56, 21st International Materials Research Congress, IMRC 2012, Cancun, Mexico, 8/12/12. https://doi.org/10.1557/opl.2012.1597
Pramatarova LD, Hikov TA, Krasteva NA, Petrik P, Dimitrova RP, Pecheva EV et al. Protein adsorption on detonation nanodiamond/polymer composite layers. In Materials Research Society Symposium Proceedings. Vol. 1479. 2013. p. 51-56 https://doi.org/10.1557/opl.2012.1597
Pramatarova, Lilyana D. ; Hikov, Todor A. ; Krasteva, Natalia A. ; Petrik, P. ; Dimitrova, Raina P. ; Pecheva, Emilia V. ; Radeva, Ekaterina I. ; Agocs, Elot ; Tsvetanov, Ivaylo G. ; Presker, Radina P. / Protein adsorption on detonation nanodiamond/polymer composite layers. Materials Research Society Symposium Proceedings. Vol. 1479 2013. pp. 51-56
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AU - Pecheva, Emilia V.

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AB - Composite layers of the detonation nanodiamond/polymer type possess a spatial organization of components with new structural features and physical properties, as well as complex functions due to the strong synergistic effects between the nanoparticles and polymer [1]. Composite layers were deposited by a plasma polymerization (PP) process of the detonation nanodiamond (DND) particles added to a hexamethyl disiloxan (HMDS) monomer [1]. The incorporation of silver ions in the polymer leads to the production of materials that are highly efficient against bacterial colonization and allows better cell adhesion and spreading. [2] For cell culture processes, fibronectin (FN) treatment is one of the commonly used approaches to enhance the cell adhesion on a surface [3]. As an integrated part of our search for improved materials for life science applications such as biomaterials and biosensors, the objective of the present study is to investigate the interaction of Ag-based composite surfaces with FN protein. Two types of composite layers, Ag-ND/PPHMDS and Ag-nano/PPHMDS were obtained by plasma polymerization of HMDS and nanoparticles of Ag and Ag-DND. The composite layers are representative of the different incorporation of the Ag in the polymer net. The structures studied, consisting of composite layers with adsorbed FN were optically characterized with Ellipsometry, Fourier Transform Infrared (FTIR) and Ultra Violet (UV) Spectroscopy as well as by stylus profiling (Talysurf). The kinetic study of the FN adsorption indicates that the process depends on the FN concentration and the exposure time as well as on the surface chemistry of the composites. Compared to the reference sample, all composite layers exhibit an indication of a stronger ability to initiate the intrinsic pathway of coagulation.

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