Protective role of air potato (Dioscorea bulbifera) of yam family in myocardial ischemic reperfusion injury

Hannah Rachel Vasanthi, Subhendu Mukherjee, Diptarka Ray, Karuppiah Shanmugasundara Pandian Jayachandran, I. Lekli, Dipak Kumar Das

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Hydroalcoholic extract of Dioscorea bulbifera (DB), a yam variety called air potato, was tested for its protective effect on myocardial ischemic/reperfusion (I/R) injury in rats due to apoptosis and necrosis. Myocardial I/R injury was induced by 30 min ischemia followed by 2 h reperfusion by perfusing isolated rat hearts with Krebs Henseilet bicarbonate (KHB) buffer in a Langendorff set up. Pretreatment of DB (150 mg kg-1 body weight) for 30 days significantly reduced myocardial infarct size and improved the ventricular function (aortic flow and coronary flow, LVDP, LVmax dp/dt). Role of DB on apoptosis was also evaluated by determining caspase 3 as well as by examining pro-apoptotic and anti-apoptotic proteins Bax and Bcl2 by Western blot analysis followed by TUNEL assay. DB also prevented I/R-mediated down regulation of survival protein Akt and HO-1. Our results indicated that Dioscorea bulbifera could ameliorate myocardial ischemia and reperfusion injury by improving ventricular function and inhibition of cardiomyocyte necrosis and apoptosis.

Original languageEnglish
Pages (from-to)278-283
Number of pages6
JournalFood and Function
Volume1
Issue number3
DOIs
Publication statusPublished - Dec 2010

Fingerprint

Dioscorea bulbifera
Dioscorea
Myocardial Reperfusion Injury
yams
Solanum tuberosum
Air
potatoes
air
apoptosis
Ventricular Function
Apoptosis
necrosis
Reperfusion
Necrosis
myocardial infarction
rats
caspase-3
myocardial ischemia
ischemia
Apoptosis Regulatory Proteins

ASJC Scopus subject areas

  • Food Science
  • Medicine(all)

Cite this

Protective role of air potato (Dioscorea bulbifera) of yam family in myocardial ischemic reperfusion injury. / Vasanthi, Hannah Rachel; Mukherjee, Subhendu; Ray, Diptarka; Pandian Jayachandran, Karuppiah Shanmugasundara; Lekli, I.; Das, Dipak Kumar.

In: Food and Function, Vol. 1, No. 3, 12.2010, p. 278-283.

Research output: Contribution to journalArticle

Vasanthi, Hannah Rachel ; Mukherjee, Subhendu ; Ray, Diptarka ; Pandian Jayachandran, Karuppiah Shanmugasundara ; Lekli, I. ; Das, Dipak Kumar. / Protective role of air potato (Dioscorea bulbifera) of yam family in myocardial ischemic reperfusion injury. In: Food and Function. 2010 ; Vol. 1, No. 3. pp. 278-283.
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