Protective mechanical ventilation does not exacerbate lung function impairment or lung inflammation following influenza A infection

Graeme R. Zosky, Vincenzo Cannizzaro, Z. Hantos, Peter D. Sly

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The degree to which mechanical ventilation induces ventilator-associated lung injury is dependent on the initial acute lung injury (ALI). Viral-induced ALI is poorly studied, and this study aimed to determine whether ALI induced by a clinically relevant infection is exacerbated by protective mechanical ventilation. Adult female BALB/c mice were inoculated with 104.5 plaque-forming units of influenza A/Mem/1/71 in 50 μl of medium or medium alone. This study used a protective ventilation strategy, whereby mice were anesthetized, tracheostomized, and mechanically ventilated for 2 h. Lung mechanics were measured periodically throughout the ventilation period using a modification of the forced oscillation technique to obtain measures of airway resistance and coefficients of tissue damping and tissue elastance. Thoracic gas volume was measured and used to obtain specific airway resistance, tissue damping, and tissue elastance. At the end of the ventilation period, a bronchoalveolar lavage sample was collected to measure inflammatory cells, macrophage inflammatory protein-2, IL-6, TNF-α, and protein leak. Influenza infection caused significant increases in inflammatory cells, protein leak, and deterioration in lung mechanics that were not exacerbated by mechanical ventilation, in contrast to previous studies using bacterial and mouse-specific viral infection. This study highlighted the importance of type and severity of lung injury in determining outcome following mechanical ventilation.

Original languageEnglish
Pages (from-to)1472-1478
Number of pages7
JournalJournal of Applied Physiology
Volume107
Issue number5
DOIs
Publication statusPublished - Nov 2009

Fingerprint

Artificial Respiration
Human Influenza
Acute Lung Injury
Pneumonia
Ventilation
Lung
Airway Resistance
Lung Injury
Infection
Mechanics
Chemokine CXCL2
Bronchoalveolar Lavage
Virus Diseases
Mechanical Ventilators
Interleukin-6
Proteins
Thorax
Gases

Keywords

  • BALB/c
  • Ventilator-associated lung injury
  • Viral infection

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Protective mechanical ventilation does not exacerbate lung function impairment or lung inflammation following influenza A infection. / Zosky, Graeme R.; Cannizzaro, Vincenzo; Hantos, Z.; Sly, Peter D.

In: Journal of Applied Physiology, Vol. 107, No. 5, 11.2009, p. 1472-1478.

Research output: Contribution to journalArticle

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