We have evaluated if mild hypothermia could have a significant protection against cerebral ischemia by its inhibition on post-ischemic apoptosis. Recent studies on cultured neuroblastoma-glioma hybrid NG108-15 cells used as a neuronal apoptotic model were examined on direct effects of mild hypothermia. Nevertheless, the mechanism of cerebral protection isn't completely known. We hypothesized that mild hypothermia may be protective against cerebral ischemia by inhibiting post-ischemia apoptosis. In this study, we used serum-deprived NG108-15 cells as the neuronal apoptotic model and examined the direct effects of mild and moderate hypothermia. Apoptosis was induced by depriving the cell culture medium of serum, which is one of the most representative methods to induce apoptosis. Cytotoxicity and the percentage of apoptotic cells were evaluated 3 days after induction of apoptosis. With induction at 37°C, cytotoxicity and the percentage of apoptotic cells were over 50 and 80%, respectively. At each temperature examined below 35°C, significant decreases in cytotoxicity and the percentage of apoptotic cells were observed. In the control group, cytotoxicity and the percentage of apoptotic cells were significantly higher at 29°C than at 37°C. We observed that mild and moderate hypothermia inhibited apoptosis.
|Number of pages||6|
|Journal||Annals of the Romanian Society for Cell Biology|
|Publication status||Published - Dec 1 2009|
- NG108-15 cells
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology