Protective effect of pure sour cherry anthocyanin extract on cytokine-induced inflammatory caco-2 monolayers

Thi Le Phuong Nguyen, Ferenc Fenyvesi, Judit Remenyik, Judit Rita Homoki, P. Gogolák, I. Bácskay, Pálma Fehér, Zoltán Ujhelyi, Gábor Vasvári, M. Vecsernyés, J. Váradi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Anthocyanins have several beneficial effects, especially on inflammatory and oxidative conditions. The pro-inflammatory cytokines, tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), induce damage in the intestinal barrier and participate in the pathogenesis of chronic bowel diseases. A number of fruits have high anthocyanin contents with strong biological activity which can support protective actions. Sour cherry (Prunus cerassus) is one of the richest fruits in anthocyanins; especially it has high content of cyanidins. The aim of this study was to test the biological effects of a pure sour cherry anthocyanin extract under inflammatory conditions on the intestinal barrier. Caco-2 monolayers were stimulated with 50 ng/mL TNF-α and 25 ng/mL IL-1β, and the protective effects of the anthocyanin extract were examined. We demonstrated the safety of 500, 50, 5 and 0.5 µM anthocyanin extracts through cell impedance measurements. The 50 µM anthocyanin extract inhibited the cytokine-induced Caco-2 permeability and the nuclear translocation of NF-κB p65 subunits. The extract significantly reduced the release of IL-6 and IL-8 production in intestinal cells and glutathione peroxidase activity stimulated by cytokines. We demonstrated, for the first time, the beneficial effects of pure sour cherry anthocyanin extract on inflammatory Caco-2 monolayers, indicating that this substance could be protective in inflammatory bowel diseases and is an excellent raw material for further applications and formulations.

Original languageEnglish
Article number861
JournalNutrients
Volume10
Issue number7
DOIs
Publication statusPublished - Jul 3 2018

Fingerprint

Prunus cerasus
Anthocyanins
protective effect
anthocyanins
cytokines
Cytokines
extracts
tumor necrosis factors
interleukin-1
Interleukin-1
Fruit
Tumor Necrosis Factor-alpha
fruits
Prunus avium
inflammatory bowel disease
Prunus
interleukin-8
impedance
Glutathione Peroxidase
Cell Extracts

Keywords

  • Anthocyanin
  • Caco-2
  • Inflammation
  • NF-κB
  • Permeability
  • Sour cherry

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Protective effect of pure sour cherry anthocyanin extract on cytokine-induced inflammatory caco-2 monolayers. / Nguyen, Thi Le Phuong; Fenyvesi, Ferenc; Remenyik, Judit; Homoki, Judit Rita; Gogolák, P.; Bácskay, I.; Fehér, Pálma; Ujhelyi, Zoltán; Vasvári, Gábor; Vecsernyés, M.; Váradi, J.

In: Nutrients, Vol. 10, No. 7, 861, 03.07.2018.

Research output: Contribution to journalArticle

Nguyen, Thi Le Phuong ; Fenyvesi, Ferenc ; Remenyik, Judit ; Homoki, Judit Rita ; Gogolák, P. ; Bácskay, I. ; Fehér, Pálma ; Ujhelyi, Zoltán ; Vasvári, Gábor ; Vecsernyés, M. ; Váradi, J. / Protective effect of pure sour cherry anthocyanin extract on cytokine-induced inflammatory caco-2 monolayers. In: Nutrients. 2018 ; Vol. 10, No. 7.
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AU - Homoki, Judit Rita

AU - Gogolák, P.

AU - Bácskay, I.

AU - Fehér, Pálma

AU - Ujhelyi, Zoltán

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AU - Vecsernyés, M.

AU - Váradi, J.

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AB - Anthocyanins have several beneficial effects, especially on inflammatory and oxidative conditions. The pro-inflammatory cytokines, tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), induce damage in the intestinal barrier and participate in the pathogenesis of chronic bowel diseases. A number of fruits have high anthocyanin contents with strong biological activity which can support protective actions. Sour cherry (Prunus cerassus) is one of the richest fruits in anthocyanins; especially it has high content of cyanidins. The aim of this study was to test the biological effects of a pure sour cherry anthocyanin extract under inflammatory conditions on the intestinal barrier. Caco-2 monolayers were stimulated with 50 ng/mL TNF-α and 25 ng/mL IL-1β, and the protective effects of the anthocyanin extract were examined. We demonstrated the safety of 500, 50, 5 and 0.5 µM anthocyanin extracts through cell impedance measurements. The 50 µM anthocyanin extract inhibited the cytokine-induced Caco-2 permeability and the nuclear translocation of NF-κB p65 subunits. The extract significantly reduced the release of IL-6 and IL-8 production in intestinal cells and glutathione peroxidase activity stimulated by cytokines. We demonstrated, for the first time, the beneficial effects of pure sour cherry anthocyanin extract on inflammatory Caco-2 monolayers, indicating that this substance could be protective in inflammatory bowel diseases and is an excellent raw material for further applications and formulations.

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