Protective effect of lactulose on dextran sulfate sodium-induced colonic inflammation in rats

G. Rumi, Ryouichi Tsubouchi, Mitsuaki Okayama, Shinichi Kato, G. Mózsik, Koji Takeuchi

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Promising results have recently been obtained with pre- and probiotic therapy in ulcerative colitis (UC). The prebiotic potential of lactulose is well established, but it has not yet been investigated in experimental colitis models. The purpose of the study was to examine the effect of lactulose on an UC model induced by 3% dextran sulfate sodium (DSS) solution added to drinking water for 7 days in male Wistar rats. Lactulose (300-1000 mg/kg) or 5-aminosalicylic acid (5-ASA; 150 mg/kg) was administered orally twice daily for 6 days. Colonic ulceration area, colon length, body weight changes, diarrhea/bloody feces, colonic mucosal myeloperoxidase activity (MPO), thiobarbituric acid reactive substances (TBARS), and histology were examined. Treatment of animals with DSS for 7 days resulted in severe colonic lesions accompanied by diarrhea, bloody feces, a decrese in body weight, shortening of the colon length, and an increase in MPO activity as well as TBARS, compared to normal rats. Lactulose treatment ameliorated DSS-induced colitis in a dose-dependent manner, and at 1000 mg/kg all of the parameters examined, except TBARS, were shown to improve significantly as compared to controls. Daily administration of 5-ASA also significantly reduced the severity of colonic lesions following DSS treatment. These results demonstrated the protectiv effect of lactulose in this rat colitis model and suggested that the background of this lactulose effect may be due to alterations of colonic microflora.

Original languageEnglish
Pages (from-to)1466-1472
Number of pages7
JournalDigestive Diseases and Sciences
Volume49
Issue number9
DOIs
Publication statusPublished - Sep 2004

Fingerprint

Lactulose
Dextran Sulfate
Inflammation
Thiobarbituric Acid Reactive Substances
Colitis
Prebiotics
Ulcerative Colitis
Feces
Peroxidase
Diarrhea
Colon
Mesalamine
Body Weight Changes
Probiotics
Therapeutics
Drinking Water
Wistar Rats
Histology
Theoretical Models
Body Weight

Keywords

  • experimental colitis
  • lactulose
  • prebiotics
  • prophylactic effect
  • rat

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Protective effect of lactulose on dextran sulfate sodium-induced colonic inflammation in rats. / Rumi, G.; Tsubouchi, Ryouichi; Okayama, Mitsuaki; Kato, Shinichi; Mózsik, G.; Takeuchi, Koji.

In: Digestive Diseases and Sciences, Vol. 49, No. 9, 09.2004, p. 1466-1472.

Research output: Contribution to journalArticle

Rumi, G. ; Tsubouchi, Ryouichi ; Okayama, Mitsuaki ; Kato, Shinichi ; Mózsik, G. ; Takeuchi, Koji. / Protective effect of lactulose on dextran sulfate sodium-induced colonic inflammation in rats. In: Digestive Diseases and Sciences. 2004 ; Vol. 49, No. 9. pp. 1466-1472.
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