Protective effect of ischaemic preconditioning on ischaemia/reperfusion- induced microvascular obstruction determined by on-line measurements of coronary pressure and blood flow in pigs

A. Pósa, Noemi Pavo, Rayyan Hemetsberger, C. Csonka, T. Csont, P. Ferdinándy, Zsolt Petrasi, C. Varga, Imre J. Pavo, F. László, Kurt Huber, Mariann Gyöngyösi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We investigated the protective effect of ischaemic preconditioning (IP) on the maintenance of coronary patency using on-line measurements of coronary pressures and blood flow in a closed-chest reperfused acute myocardial infarction (MI) model in pigs. Catheter-based 90-min occlusion followed by 60-min reperfusion of the left anterior descending coronary artery (LAD) was performed in anesthetised pigs (MI group). IP was applied (IP group) through two cycles of 5-min occlusion and 5-min reperfusion of the LAD before MI induction. Coronary patency was determined by measurements of coronary wedge pressure, collateral fractional flow reserve (FFRcoll), collateral pressure index (CPI) and absolute coronary blood flow (CBF). Inducible and constitutive nitric oxide synthase (iNOS/cNOS) activities and expressions were determined in the myocardium. Plasma levels of myeloperoxidase (MPO, index of activated leukocytes) and mean platelet volume (MPV, index of activated platelets) were measured. IP resulted in significantly lower levels of MPO (0.52 ± 0.19 vs. 1.05 ± 0.24 U/l, p

Original languageEnglish
Pages (from-to)450-460
Number of pages11
JournalThrombosis and Haemostasis
Volume103
Issue number2
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Ischemic Preconditioning
Reperfusion
Swine
Ischemia
Blood Pressure
Myocardial Infarction
Mean Platelet Volume
Pulmonary Wedge Pressure
Nitric Oxide Synthase Type II
Peroxidase
Coronary Vessels
Myocardium
Leukocytes
Thorax
Blood Platelets
Catheters
Maintenance
Pressure

Keywords

  • Collateral circulation
  • Ischaemia/reperfusion
  • Nitric oxide

ASJC Scopus subject areas

  • Hematology

Cite this

Protective effect of ischaemic preconditioning on ischaemia/reperfusion- induced microvascular obstruction determined by on-line measurements of coronary pressure and blood flow in pigs. / Pósa, A.; Pavo, Noemi; Hemetsberger, Rayyan; Csonka, C.; Csont, T.; Ferdinándy, P.; Petrasi, Zsolt; Varga, C.; Pavo, Imre J.; László, F.; Huber, Kurt; Gyöngyösi, Mariann.

In: Thrombosis and Haemostasis, Vol. 103, No. 2, 02.2010, p. 450-460.

Research output: Contribution to journalArticle

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