Protective effect of Cassia fistula fruit extract against bromobenzene-induced liver injury in mice

Heibatullah Kalantari, Mohammadtaha Jalali, Amir Jalali, Masood Mahdavinia, Abobakr Salimi, Bela Juhasz, Arpad Tosaki, Rudolf Gesztelyi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In the present study, hepatoprotective effect of Cassia fistula fruit extract was investigated in mice. Animals were divided into six groups receiving normal saline (1), bromobenzene (460 mg/kg) alone (2) and together with increasing doses (200, 400, 600, 800 mg/kg) of a crude hydro-alcoholic extract of Cassia fistula fruit (3-6, respectively). All administrations were carried out orally, daily, for 10 days. On the 11th day, animals were sacrificed. Serum activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma glutamyl transpeptidase (γGT) were determined; serum levels of direct and total bilirubin were measured; furthermore, livers were prepared for histological examination. Our results showed that bromobenzene treatment alone elicited a significant increase in activities of AST, ALT, ALP (but not γGT), and it significantly elevated the levels of direct and total bilirubin. Co-treatment with Cassia fistula fruit extract, however, significantly and dose-dependently decreased the above-mentioned enzyme activities (with exception of γGT) and bilirubin levels, producing a recovery to the naive state. The protective effect of Cassia fistula fruit extract against liver injury evoked by bromobenzene was confirmed by histological examination as well. In conclusion, the Cassia fistula fruit extract has significant hepatoprotective effect in our murine model.

Original languageEnglish
Pages (from-to)1039-1044
Number of pages6
JournalHuman and Experimental Toxicology
Volume30
Issue number8
DOIs
Publication statusPublished - Aug 2011

Keywords

  • Cassia fistula
  • bromobenzene
  • hepatic toxicology
  • liver
  • mouse

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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