Protective effect of carboxyethylgermanium sesquioxide (Ge-132) on superoxide generation by60Co-irradiated leukocytes

L. Prónai, Shigeru Arimori

Research output: Contribution to journalArticle

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Abstract

The carboxyethylgermanium sesquioxide, Ge-132, is an organogermanium compound which has been shown to modulate leukocyte functions. In this study, we examined the effect of Ge-132 on the generation of superoxide radicals (O2 -) either from leukocytes or in cell-free system, employing the highly sensitive 2-methyl-6-[p-methoxy-phenyl]-3,7-dihydroimidazo[1,2-alpha] pyrazin-3-one (MCLA) -dependent chemiluminescence method and the specific electron spin resonance/spin trapping method, respectively. In addition, the in vitro protective effect of Ge-132 on the leukocytes irradiated with60Co was studied. The incubation with Ge-132 resulted in an increase in basal O2 - release of intact leukocytes, but had no effect on OZ generation from leukocytes stimulated with phorbol myristate acetate (PMA). Irradiation with60Co decreased the Oz generation from leukocytes in a dose-dependent manner. Ge-132 had no effect on basal O2 - release from60Co-irradiated leukocytes, but it prevented the decrease in PMA-stimulated O2 - generation by irradiated leukocytes. Ge-132 itself had no superoxide scavenging activity in cell-free system. On the other hand, higher concentrations of Ge-132 had decreasing effects on both basal O2 -1 release and PMA-stimulated O2 - generation from leukocytes, but they did not affect leukocyte viability. Above results indicate that 1) Ge-132 can stimulate the basal O2 - release from leukocytes, 2) Ge-132 can prevent the decrease of O2 - generation by60Co-irradiated leukocytes, 3) in higher concentrations, Ge-132 may have a membrane stabilizing effect.

Original languageEnglish
Pages (from-to)273-279
Number of pages7
JournalBiotherapy
Volume3
Issue number3
DOIs
Publication statusPublished - Jul 1991

Fingerprint

Superoxides
Leukocytes
Tetradecanoylphorbol Acetate
Cell-Free System
proxigermanium
Spin Trapping
Electron Spin Resonance Spectroscopy
Luminescence
Membranes

Keywords

  • Co-irradiation
  • Ge-132
  • leukocyte
  • superoxide generation
  • superoxide scavenging activity

ASJC Scopus subject areas

  • Pharmacology

Cite this

Protective effect of carboxyethylgermanium sesquioxide (Ge-132) on superoxide generation by60Co-irradiated leukocytes. / Prónai, L.; Arimori, Shigeru.

In: Biotherapy, Vol. 3, No. 3, 07.1991, p. 273-279.

Research output: Contribution to journalArticle

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