Proteasomal ATPases are associated with rDNA: The ubiquitin proteasome system plays a direct role in RNA polymerase I transcription

K. Fátyol, Ingrid Grummt

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Significant amount of data have accumulated in the last several years pointing to the essential role of the ubiquitin proteasome system in the regulation of RNA polymerase II transcription; however, its involvement in RNA polymerase I transcription has remained largely unexplored. In this study, we demonstrate that proteasome activity is required for pre-rRNA synthesis. We can detect the association of proteasomal ATPases with both the rDNA promoter and coding region. Additionally, we show that the RNA polymerase I associated transcription factor, TIF-IA interacts with proteasomal ATPases, representing a potential link via which proteasomes and/or proteasome related complexes are recruited to rRNA genes. In summary, our findings suggest that the ubiquitin proteasome system is directly involved in RNA polymerase I transcription in analogy to the RNA polymerase II system.

Original languageEnglish
Pages (from-to)850-859
Number of pages10
JournalBiochimica et Biophysica Acta - Gene Regulatory Mechanisms
Volume1779
Issue number12
DOIs
Publication statusPublished - Dec 2008

Fingerprint

RNA Polymerase I
Proteasome Endopeptidase Complex
Transcription
Ubiquitin
Ribosomal DNA
Adenosine Triphosphatases
RNA Polymerase II
Pol1 Transcription Initiation Complex Proteins
RNA Precursors
rRNA Genes
Genetic Promoter Regions
Transcription Factors
Genes
Association reactions

Keywords

  • RNA polymerase I
  • Transcription
  • Ubiquitin proteasome system

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

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