Typical sources of error in the UV spectroscopy of DNA near and below 200 nm are pointed out. In the case of native DNA a procedure for extending the wavelenght range and for analysing the spectra is proposed. As illustration, spectra for DNAs of various origin recorded in the 164-350 nm range at room temperature and at 110 K are presented.
- DNA thin films
- vacuum-ultraviolet spectroscopy
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