Properties of non-neurovirulent plaque-forming mutants of Newcaslte disease virus.

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Abstract

The mesogenic Hertfordshire (H) strain, a vaccine strain of Newcastle disease virus, represents a heterogeneous virus population differing in physical, chemical, and biological properties. Small (S) and large (L) plaque-forming mutants were isolted from strain H in chick embryo fibroblast cell cultures. Part of the S mutants lacked neurovirulence, with an intracerebral pathogenicity index for day-old chicks of 0 to 0.46 vs 1.0 to 1.4 for L mutants. S mutants grew only to a very low titer in the brain of day-old chicks and cleared from the brain by the sixth day, whereas L mutants killed the chicks after a 1000-fold titer rise. The S mutants caused a complete cell destruction in fibroblast culture by 12 hours, but they had 10--20 times lower virus yields than the L mutants. The infective titers of S and L mutants grown in allantoic cells were identical, but the infectivity to hemagglutinin ratio was 10 to 50 times lower for S than for L. The thermostability of infectivity and hemagglutinin varied with the different mutants. As for the immunogenicity of the mutants, the minimum dose of S mutants inducing full protection by subcutaneous inoculation was 10(6) plaque-forming units per chicken, thus being about 100 times less immunogenic than either the L mutants or the parent strain H.

Original languageEnglish
Pages (from-to)126-134
Number of pages9
JournalAvian Diseases
Volume20
Issue number1
Publication statusPublished - Jan 1976

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Hemagglutinins
Virus Diseases
Fibroblasts
Viruses
Newcastle disease virus
viruses
mutants
Brain
Chick Embryo
Virulence
Chickens
Vaccines
Cell Culture Techniques
chicks
Population
pathogenicity
hemagglutinins
fibroblasts
brain
thermal stability

ASJC Scopus subject areas

  • Cancer Research
  • Immunology
  • veterinary(all)

Cite this

Properties of non-neurovirulent plaque-forming mutants of Newcaslte disease virus. / Lomniczi, B.

In: Avian Diseases, Vol. 20, No. 1, 01.1976, p. 126-134.

Research output: Contribution to journalArticle

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