Properties and antimicrobial susceptibility of trueperella pyogenes isolated from bovine mastitis in China

Rashad Alkasir, Jianfang Wang, Jian Gao, Tariq Ali, Limei Zhang, Ottó Szenci, Árpád Csaba Bajcsy, Bo Han

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Trueperella (T.) pyogenes is an opportunistic pathogen that causes suppurative diseases in domestic animals. In this work, the properties, pathogenesis and phenotypic diversity of T. pyogenes isolates from bovine mastitis were studied. Both pyolysin (plo) and collagen-binding protein (cbp) virulence factor genes were detected by PCR in all T. pyogenes isolates (n = 50). Using the tissue culture plate method, 90% of T. pyogenes isolates were able to form biofilms. The minimum inhibitory concentrations (MICs) of 13 antimicrobials against T. pyogenes isolates were determined. High susceptibility was observed to rifampin (96%), ampicillin (94%), ciprofloxacin (94%), and penicillin (92%), while low susceptibility was found to trimethoprim-sulphamethoxazole (10%) and bacitracin (2%). The intracellular assay revealed that T. pyogenes isolates had different cytopathogenic effects on cells. The high percentage (28.6%) of T. pyogenes isolates suggests that this bacterium is an important contributor to mastitis. Moreover, the high occurrence of multidrug resistance, biofilm production, intracellular survival, and the temporal dynamics of T. pyogenes interactions are key factors for a better understanding of how immunity acts on infections with these bacteria and how they evade immune surveillance, thus highlighting the need for the prudent use of antimicrobial agents in veterinary medicine.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalActa Veterinaria Hungarica
Volume64
Issue number1
DOIs
Publication statusPublished - Mar 1 2016

Fingerprint

Bovine Mastitis
Arcanobacterium pyogenes
bovine mastitis
Biofilms
China
anti-infective agents
Bacitracin
Bacteria
Sulfamethoxazole
Trimethoprim
Veterinary Medicine
Mastitis
Domestic Animals
Microbial Sensitivity Tests
Multiple Drug Resistance
Virulence Factors
Ampicillin
Rifampin
Ciprofloxacin
Anti-Infective Agents

Keywords

  • Antimicrobial susceptibility
  • Biofilm
  • Bovine mastitis
  • Trueperella pyogenes
  • Virulence factor

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Properties and antimicrobial susceptibility of trueperella pyogenes isolated from bovine mastitis in China. / Alkasir, Rashad; Wang, Jianfang; Gao, Jian; Ali, Tariq; Zhang, Limei; Szenci, Ottó; Bajcsy, Árpád Csaba; Han, Bo.

In: Acta Veterinaria Hungarica, Vol. 64, No. 1, 01.03.2016, p. 1-12.

Research output: Contribution to journalArticle

Alkasir, Rashad ; Wang, Jianfang ; Gao, Jian ; Ali, Tariq ; Zhang, Limei ; Szenci, Ottó ; Bajcsy, Árpád Csaba ; Han, Bo. / Properties and antimicrobial susceptibility of trueperella pyogenes isolated from bovine mastitis in China. In: Acta Veterinaria Hungarica. 2016 ; Vol. 64, No. 1. pp. 1-12.
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