Promoter hypermethylation-related reduced somatostatin production promotes uncontrolled cell proliferation in colorectal cancer

Katalin Leiszter, F. Sípos, O. Galamb, T. Krenács, G. Verès, Barna Wichmann, István Furi, Alexandra Kalmár, Árpá V. Patai, Kinga Tóth, Gábor Valcz, Z. Tulassay, B. Molnár

Research output: Contribution to journalArticle

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Abstract

Background: Somatostatin (SST) has anti-proliferative and pro-apoptotic effects. Our aims were to analyze and compare the SST expression during normal aging and colorectal carcinogenesis at mRNA and protein levels. Furthermore, we tested the methylation status of SST in biopsy samples, and the cell growth inhibitory effect of the SST analogue octreotide in human colorectal adenocarcinoma cell line. Methods: Colonic samples were collected from healthy children (n1 = 6), healthy adults (n2 = 41) and colorectal cancer patients (CRCs) (n3 = 34) for SST mRNA expression analysis, using HGU133 Plus2.0 microarrays. Results were validated both on original (n1 = 6; n2 = 6; n3 = 6) and independent samples ((n1 = 6; n2 = 6; n3 = 6) by real-time PCR. SST expressing cells were detected by immunohistochemistry on colonic biopsy samples (n1 = 14; n2 = 20; n3 = 23). The effect of octreotide on cell growth was tested on Caco-2 cell line. SST methylation percentage in biopsy samples (n1 = 5; n2 = 5; n3 = 9) was defined using methylation-sensitive restriction enzyme digestion. Results: In case of normal aging SST mRNA expression did not alter, but decreased in cancer (p

Original languageEnglish
Article numbere0118332
JournalPLoS One
Volume10
Issue number2
DOIs
Publication statusPublished - Feb 27 2015

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somatostatin
Cell proliferation
Somatostatin
colorectal neoplasms
Colorectal Neoplasms
cell proliferation
promoter regions
Cell Proliferation
Methylation
Biopsy
Octreotide
octreotide
methylation
biopsy
Cell growth
Messenger RNA
Aging of materials
Cell Line
cell growth
sampling

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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Promoter hypermethylation-related reduced somatostatin production promotes uncontrolled cell proliferation in colorectal cancer. / Leiszter, Katalin; Sípos, F.; Galamb, O.; Krenács, T.; Verès, G.; Wichmann, Barna; Furi, István; Kalmár, Alexandra; Patai, Árpá V.; Tóth, Kinga; Valcz, Gábor; Tulassay, Z.; Molnár, B.

In: PLoS One, Vol. 10, No. 2, e0118332, 27.02.2015.

Research output: Contribution to journalArticle

Leiszter, Katalin ; Sípos, F. ; Galamb, O. ; Krenács, T. ; Verès, G. ; Wichmann, Barna ; Furi, István ; Kalmár, Alexandra ; Patai, Árpá V. ; Tóth, Kinga ; Valcz, Gábor ; Tulassay, Z. ; Molnár, B. / Promoter hypermethylation-related reduced somatostatin production promotes uncontrolled cell proliferation in colorectal cancer. In: PLoS One. 2015 ; Vol. 10, No. 2.
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