Prolonged histamine deficiency in histidine decarboxylase gene knockout mice affects Leydig cell function

Carolina Mondillo, A. Falus, Omar Pignataro, Erna Pap

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The present study focuses on histaminergic regulation of Leydig cell physiology, since limited information is available so far. To evaluate the dependency of Leydig cells on histamine (HA), we performed experiments using highly purified Leydig cells in culture, isolated from wild type (WT) and histidine decarboxylase (Hdc) gene knockout (HDC KO)-so HA-deprived-mice. HDC KO Leydig cells showed lower basal and human choriogonadotropin (hCG)-induced testosterone production compared to WT Leydig cells, presumably due to altered P450scc gene (Cyp11a1) expression levels. Moreover, in HDC KO cells, hCG did not increase basal expression levels of HA H1 and H2 receptor genes, while the hormone showed a significant inducing effect in WT cells. Based on these findings, we propose that prolonged HA deficiency in HDC KO mice affects various aspects of Leydig cell physiology, most importantly the response to hCG, providing definite evidence that HA plays a role as direct modulator of Leydig cell function and steroid synthesis in the testis. Also, the results presented herein constitute the first molecular evidence for the expression of HA H1 and H2 receptor subtypes in isolated Leydig cells.

Original languageEnglish
Pages (from-to)86-91
Number of pages6
JournalJournal of Andrology
Volume28
Issue number1
DOIs
Publication statusPublished - Jan 2007

Fingerprint

Histidine Decarboxylase
Gene Knockout Techniques
Leydig Cells
Knockout Mice
Histamine
Chorionic Gonadotropin
Cell Physiological Phenomena
Histamine H1 Receptors
Histamine H2 Receptors
Testosterone
Testis
Cell Culture Techniques
Steroids
Hormones
Gene Expression

Keywords

  • Human choriogonadotropin
  • Steroidogenesis

ASJC Scopus subject areas

  • Endocrinology

Cite this

Prolonged histamine deficiency in histidine decarboxylase gene knockout mice affects Leydig cell function. / Mondillo, Carolina; Falus, A.; Pignataro, Omar; Pap, Erna.

In: Journal of Andrology, Vol. 28, No. 1, 01.2007, p. 86-91.

Research output: Contribution to journalArticle

Mondillo, Carolina ; Falus, A. ; Pignataro, Omar ; Pap, Erna. / Prolonged histamine deficiency in histidine decarboxylase gene knockout mice affects Leydig cell function. In: Journal of Andrology. 2007 ; Vol. 28, No. 1. pp. 86-91.
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