Progressive increase of human papillomavirus carriage rates in potentially malignant and malignant oral disorders with increasing malignant potential

K. Szarka, I. Tar, E. Feher, T. Gall, A. Kis, E. D. Toth, R. Boda, I. Márton, L. Gergely

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Introduction: We investigated the potential role of human papillomaviruses (HPVs) in potentially malignant oral disorders, oral leukoplakia (OL) and oral lichen planus (OLP), and in oral squamous cell cancer (OSCC) in an Eastern Hungarian population with a high incidence of OSCC. Methods: Excised tumor samples (65 OSCC patients) and exfoliated cells from potentially malignant lesions (from 44 and 119 patients with OL and OLP, respectively) as well as from healthy controls (72 individuals) were analysed. OLPs were classified based on clinical appearance, 61 patients had erosive-atrophic lesions (associated with higher malignancy risk, EA-OLP) and 58 had non-erosive non-atrophic lesions (with lower risk of becoming malignant, non-EA-OLP), respectively. Exfoliated cells collected from apparently healthy mucosa accompanied each lesion sample. HPV was detected by MY/GP polymerase chain reaction (PCR) and genotyped by restriction analysis of amplimers. Copy numbers in lesions were determined using real-time PCR. Prevalence rates, copy number distributions, and association with risk factors and diseases were analysed using chi-square test, t-test, and logistic regression, respectively. Results: We detected HPVs significantly more frequently in lesions than in controls (P ≤ 0.001 in all comparisons). HPV prevalence increased gradually with increasing severity of lesions (32.8, 40.9, and 47.7% in OLP, OL, and OSCC, respectively). Copy number distribution patterns roughly corresponded to prevalence rates, but OLP and OL were comparable. HPV prevalence differed significantly between EA-OLP and non-EA-OLP groups (42.6 vs. 22.4%); EA-OLP group showed a prevalence similar to that found in OL. Conclusion: HPVs may be involved in the development or progression of not only OSCC but also of potentially malignant oral lesions.

Original languageEnglish
Pages (from-to)314-318
Number of pages5
JournalOral Microbiology and Immunology
Volume24
Issue number4
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Oral Lichen Planus
Oral Leukoplakia
Squamous Cell Neoplasms
Mouth Neoplasms
Chi-Square Distribution
Real-Time Polymerase Chain Reaction
Neoplasms
Mucous Membrane
Logistic Models

Keywords

  • Human papillomavirus
  • Oral carcinogenesis
  • Oral leukoplakia
  • Oral lichen planus
  • Oral squamous cell cancer

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)
  • Microbiology
  • Dentistry(all)

Cite this

Progressive increase of human papillomavirus carriage rates in potentially malignant and malignant oral disorders with increasing malignant potential. / Szarka, K.; Tar, I.; Feher, E.; Gall, T.; Kis, A.; Toth, E. D.; Boda, R.; Márton, I.; Gergely, L.

In: Oral Microbiology and Immunology, Vol. 24, No. 4, 08.2009, p. 314-318.

Research output: Contribution to journalArticle

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abstract = "Introduction: We investigated the potential role of human papillomaviruses (HPVs) in potentially malignant oral disorders, oral leukoplakia (OL) and oral lichen planus (OLP), and in oral squamous cell cancer (OSCC) in an Eastern Hungarian population with a high incidence of OSCC. Methods: Excised tumor samples (65 OSCC patients) and exfoliated cells from potentially malignant lesions (from 44 and 119 patients with OL and OLP, respectively) as well as from healthy controls (72 individuals) were analysed. OLPs were classified based on clinical appearance, 61 patients had erosive-atrophic lesions (associated with higher malignancy risk, EA-OLP) and 58 had non-erosive non-atrophic lesions (with lower risk of becoming malignant, non-EA-OLP), respectively. Exfoliated cells collected from apparently healthy mucosa accompanied each lesion sample. HPV was detected by MY/GP polymerase chain reaction (PCR) and genotyped by restriction analysis of amplimers. Copy numbers in lesions were determined using real-time PCR. Prevalence rates, copy number distributions, and association with risk factors and diseases were analysed using chi-square test, t-test, and logistic regression, respectively. Results: We detected HPVs significantly more frequently in lesions than in controls (P ≤ 0.001 in all comparisons). HPV prevalence increased gradually with increasing severity of lesions (32.8, 40.9, and 47.7{\%} in OLP, OL, and OSCC, respectively). Copy number distribution patterns roughly corresponded to prevalence rates, but OLP and OL were comparable. HPV prevalence differed significantly between EA-OLP and non-EA-OLP groups (42.6 vs. 22.4{\%}); EA-OLP group showed a prevalence similar to that found in OL. Conclusion: HPVs may be involved in the development or progression of not only OSCC but also of potentially malignant oral lesions.",
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AU - Tar, I.

AU - Feher, E.

AU - Gall, T.

AU - Kis, A.

AU - Toth, E. D.

AU - Boda, R.

AU - Márton, I.

AU - Gergely, L.

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