Programmed cell death in Datura innoxia Mill. callus cultures cultivated in light and in dark

I. László, É Szke, E. Tyihák, B. Szende

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

It has been established that dimedone in solid culture medium influenced the growth of Datura innoxia Mill. callus tissues and the apoptotic processes of cells. This formaldehyde (HCHO) capture reagent appears to modify the metabolism of plant cells, resulting in quantitative changes in the apoptotic index (Ai). Apoptotic cells were detected in six-week-old callus tissues by the TUNEL reaction. The amount of TUNEL positive nuclei showed a characteristic spatial distribution. Enhanced DNA fragmentation was observed in the cell layers close to the surface of the cultures. Elevated Ai was determined in cultures grown in the dark compared to the tissues grown in the light. High doses of dimedone considerably decreased apoptosis in tissue cultures under both light and dark conditions.

Original languageEnglish
Pages (from-to)231-236
Number of pages6
JournalPlant Growth Regulation
Volume33
Issue number3
DOIs
Publication statusPublished - 2001

Fingerprint

Datura
Bony Callus
callus culture
Cell Death
apoptosis
Light
In Situ Nick-End Labeling
callus
cells
DNA fragmentation
Plant Cells
DNA Fragmentation
formaldehyde
Formaldehyde
tissue culture
Culture Media
culture media
spatial distribution
Apoptosis
metabolism

Keywords

  • Apoptosis
  • Callus culture
  • Datura innoxia Mill.
  • Formaldehyde
  • TUNEL reaction

ASJC Scopus subject areas

  • Plant Science

Cite this

Programmed cell death in Datura innoxia Mill. callus cultures cultivated in light and in dark. / László, I.; Szke, É; Tyihák, E.; Szende, B.

In: Plant Growth Regulation, Vol. 33, No. 3, 2001, p. 231-236.

Research output: Contribution to journalArticle

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