Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K Pathway

Tor Erik Rusten, Karine Lindmo, Gábor Juhász, Miklós Sass, Per O. Seglen, Andreas Brech, Harald Stenmark

Research output: Contribution to journalArticle

Abstract

Eukaryotic cells catabolize their own cytoplasm by autophagy in response to amino acid starvation and inductive signals during programmed tissue remodeling and cell death. The Tor and PI3K signaling pathways have been shown to negatively control autophagy in eukaryotes, but the mechanisms that link these effectors to overall animal development and nutritional status in multicellular organisms remain poorly understood. Here, we reveal a complex regulation of programmed and starvation-induced autophagy in the Drosophila fat body. Gain-of-function genetic analysis indicated that ecdysone receptor signaling induces programmed autophagy whereas PI3K signaling represses programmed autophagy. Genetic interaction studies showed that ecdysone signaling downregulates PI3K signaling and that this represents the effector mechanism for induction of programmed autophagy. Hence, these studies link hormonal induction of autophagy to the regulatory function of the PI3K signaling pathway in vivo.

Original languageEnglish
Pages (from-to)179-192
Number of pages14
JournalDevelopmental Cell
Volume7
Issue number2
DOIs
Publication statusPublished - Aug 2004

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Ecdysone
Fat Body
Autophagy
Phosphatidylinositol 3-Kinases
Drosophila
Fats
Starvation
Cell death
Eukaryotic Cells
Eukaryota
Nutritional Status
Animals
Cytoplasm
Tissue
Cell Death
Down-Regulation
Amino Acids

ASJC Scopus subject areas

  • Developmental Biology

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Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K Pathway. / Rusten, Tor Erik; Lindmo, Karine; Juhász, Gábor; Sass, Miklós; Seglen, Per O.; Brech, Andreas; Stenmark, Harald.

In: Developmental Cell, Vol. 7, No. 2, 08.2004, p. 179-192.

Research output: Contribution to journalArticle

Rusten, Tor Erik ; Lindmo, Karine ; Juhász, Gábor ; Sass, Miklós ; Seglen, Per O. ; Brech, Andreas ; Stenmark, Harald. / Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K Pathway. In: Developmental Cell. 2004 ; Vol. 7, No. 2. pp. 179-192.
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