Prognostic value of DLGAP5 in colorectal cancer

Vittorio Branchi, Sebastián A. García, Praveen Radhakrishnan, B. Györffy, Barbara Hissa, Martin Schneider, Christoph Reißfelder, Sebastian Schölch

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: DLG7 (disc large homolog 7) is a microtubule-associated protein encoded by DLGAP5 (DLG associated protein 5) gene and has an important role during spindle assembly. Spindle assembly deregulation is a well-known cause of genomic instability. The aim of this study was to investigate the influence of DLGAP5 expression on survival and to evaluate its potential use as a biomarker in colorectal cancer (CRC). Methods: DLGAP5 expression was measured in the primary tumor and corresponding normal mucosa samples from 109 patients with CRC and correlated to clinical and pathological data. The results were validated in a second, publically available patient cohort. Molecular effects of DLG7/DLGAP5 in CRC were analyzed via functional assays in knockdown cell lines. Results: DLGAP5 downregulation led to a significant reduction of the invasion and migration potential in CRC. In addition, DLGAP5 expression correlates with nodal status and advanced UICC stage (III–IV).Subgroup analyses revealed a correlation between DLGAP5 overexpression and poor survival in patients with non-metastatic disease (M0). Furthermore, overexpression of DLGAP5 is associated with worse overall survival in distinct molecular CRC subtypes. Conclusions: The results of this study suggest the importance of DLGAP5 in defining a more aggressive CRC phenotype. DLG7/DLGAP5 represents a potential biomarker for CRC in molecular subgroups of CRC.

Original languageEnglish
Pages (from-to)1455-1465
Number of pages11
JournalInternational Journal of Colorectal Disease
Volume34
Issue number8
DOIs
Publication statusPublished - Aug 1 2019

Fingerprint

Colorectal Neoplasms
Survival
Biomarkers
SAPAP proteins
Microtubule-Associated Proteins
Genomic Instability
Mucous Membrane
Down-Regulation
Phenotype
Cell Line
Genes

Keywords

  • Colorectal cancer
  • DLG7
  • DLGAP5
  • Metastasis
  • Survival

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Branchi, V., García, S. A., Radhakrishnan, P., Györffy, B., Hissa, B., Schneider, M., ... Schölch, S. (2019). Prognostic value of DLGAP5 in colorectal cancer. International Journal of Colorectal Disease, 34(8), 1455-1465. https://doi.org/10.1007/s00384-019-03339-6

Prognostic value of DLGAP5 in colorectal cancer. / Branchi, Vittorio; García, Sebastián A.; Radhakrishnan, Praveen; Györffy, B.; Hissa, Barbara; Schneider, Martin; Reißfelder, Christoph; Schölch, Sebastian.

In: International Journal of Colorectal Disease, Vol. 34, No. 8, 01.08.2019, p. 1455-1465.

Research output: Contribution to journalArticle

Branchi, V, García, SA, Radhakrishnan, P, Györffy, B, Hissa, B, Schneider, M, Reißfelder, C & Schölch, S 2019, 'Prognostic value of DLGAP5 in colorectal cancer', International Journal of Colorectal Disease, vol. 34, no. 8, pp. 1455-1465. https://doi.org/10.1007/s00384-019-03339-6
Branchi V, García SA, Radhakrishnan P, Györffy B, Hissa B, Schneider M et al. Prognostic value of DLGAP5 in colorectal cancer. International Journal of Colorectal Disease. 2019 Aug 1;34(8):1455-1465. https://doi.org/10.1007/s00384-019-03339-6
Branchi, Vittorio ; García, Sebastián A. ; Radhakrishnan, Praveen ; Györffy, B. ; Hissa, Barbara ; Schneider, Martin ; Reißfelder, Christoph ; Schölch, Sebastian. / Prognostic value of DLGAP5 in colorectal cancer. In: International Journal of Colorectal Disease. 2019 ; Vol. 34, No. 8. pp. 1455-1465.
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