Progesterone-induced blocking factor (PIBF) and trophoblast invasiveness

E. Miko, M. Halasz, B. Jericevic-Mulac, L. Wicherek, P. Arck, G. Arató, J. Skret Magierlo, D. Rukavina, J. Szekeres-Barthó

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Controlled trophoblast invasion is a key process during human placentation and a prerequisite for successful pregnancy. Progesterone is one of the factors to regulate trophoblast invasiveness. Progesterone-induced blocking factor (PIBF) is a progesterone-induced molecule expressed by the trophoblast, and also by tumors. The distribution of PIBF within the first-trimester decidua coincides with sites of trophoblast invasion. Another molecule that has been implicated in the control of trophoblast invasiveness is placental leptin. Leptin inhibits the secretion of progesterone by cytotrophoblast. The aim of this work was to investigate the possible interaction of PIBF and leptins in regulating trophoblast invasion. Paraffin-embedded sections from normal first-trimester placentae, partial moles, complete moles, and choriocarcinomas were reacted with PIBF, leptin, and leptin receptor specific antibodies. PIBF-deficient trophoblast cells were generated using siRNA and leptin receptor was detected on Western blot analysis. The lysates of PIBF-treated cells were used for detecting leptin expression in a protein array. PIBF was expressed in both normal first-trimester villous trophoblast and in partial mole. Compared with this, PIBF expression was markedly decreased in complete mole and absent in choriocarcinoma. Neither leptinR nor leptin were detected in partial mole, whereas both of these molecules were present in complete mole and choriocarcinoma. Leptin receptor expression was upregulated in PIBF-deficient cells, while leptin expression was decreased in PIBF-treated cells. These data suggest that PIBF affects the expression of leptin and its receptor, and that PIBF expression is inversely related to trophoblast invasiveness.

Original languageEnglish
Pages (from-to)50-57
Number of pages8
JournalJournal of Reproductive Immunology
Volume90
Issue number1
DOIs
Publication statusPublished - Jun 2011

Fingerprint

Trophoblasts
Progesterone
Leptin
Leptin Receptors
Choriocarcinoma
First Pregnancy Trimester
blocking factor
Trophoblastic Neoplasms
Decidua
Placentation
Protein Array Analysis
Paraffin
Placenta
Small Interfering RNA

Keywords

  • Invasion
  • Leptin
  • PIBF

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Progesterone-induced blocking factor (PIBF) and trophoblast invasiveness. / Miko, E.; Halasz, M.; Jericevic-Mulac, B.; Wicherek, L.; Arck, P.; Arató, G.; Skret Magierlo, J.; Rukavina, D.; Szekeres-Barthó, J.

In: Journal of Reproductive Immunology, Vol. 90, No. 1, 06.2011, p. 50-57.

Research output: Contribution to journalArticle

Miko, E, Halasz, M, Jericevic-Mulac, B, Wicherek, L, Arck, P, Arató, G, Skret Magierlo, J, Rukavina, D & Szekeres-Barthó, J 2011, 'Progesterone-induced blocking factor (PIBF) and trophoblast invasiveness', Journal of Reproductive Immunology, vol. 90, no. 1, pp. 50-57. https://doi.org/10.1016/j.jri.2011.03.005
Miko E, Halasz M, Jericevic-Mulac B, Wicherek L, Arck P, Arató G et al. Progesterone-induced blocking factor (PIBF) and trophoblast invasiveness. Journal of Reproductive Immunology. 2011 Jun;90(1):50-57. https://doi.org/10.1016/j.jri.2011.03.005
Miko, E. ; Halasz, M. ; Jericevic-Mulac, B. ; Wicherek, L. ; Arck, P. ; Arató, G. ; Skret Magierlo, J. ; Rukavina, D. ; Szekeres-Barthó, J. / Progesterone-induced blocking factor (PIBF) and trophoblast invasiveness. In: Journal of Reproductive Immunology. 2011 ; Vol. 90, No. 1. pp. 50-57.
@article{19e859f617d1453cb9ee23bb192d350c,
title = "Progesterone-induced blocking factor (PIBF) and trophoblast invasiveness",
abstract = "Controlled trophoblast invasion is a key process during human placentation and a prerequisite for successful pregnancy. Progesterone is one of the factors to regulate trophoblast invasiveness. Progesterone-induced blocking factor (PIBF) is a progesterone-induced molecule expressed by the trophoblast, and also by tumors. The distribution of PIBF within the first-trimester decidua coincides with sites of trophoblast invasion. Another molecule that has been implicated in the control of trophoblast invasiveness is placental leptin. Leptin inhibits the secretion of progesterone by cytotrophoblast. The aim of this work was to investigate the possible interaction of PIBF and leptins in regulating trophoblast invasion. Paraffin-embedded sections from normal first-trimester placentae, partial moles, complete moles, and choriocarcinomas were reacted with PIBF, leptin, and leptin receptor specific antibodies. PIBF-deficient trophoblast cells were generated using siRNA and leptin receptor was detected on Western blot analysis. The lysates of PIBF-treated cells were used for detecting leptin expression in a protein array. PIBF was expressed in both normal first-trimester villous trophoblast and in partial mole. Compared with this, PIBF expression was markedly decreased in complete mole and absent in choriocarcinoma. Neither leptinR nor leptin were detected in partial mole, whereas both of these molecules were present in complete mole and choriocarcinoma. Leptin receptor expression was upregulated in PIBF-deficient cells, while leptin expression was decreased in PIBF-treated cells. These data suggest that PIBF affects the expression of leptin and its receptor, and that PIBF expression is inversely related to trophoblast invasiveness.",
keywords = "Invasion, Leptin, PIBF",
author = "E. Miko and M. Halasz and B. Jericevic-Mulac and L. Wicherek and P. Arck and G. Arat{\'o} and {Skret Magierlo}, J. and D. Rukavina and J. Szekeres-Barth{\'o}",
year = "2011",
month = "6",
doi = "10.1016/j.jri.2011.03.005",
language = "English",
volume = "90",
pages = "50--57",
journal = "Journal of Reproductive Immunology",
issn = "0165-0378",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Progesterone-induced blocking factor (PIBF) and trophoblast invasiveness

AU - Miko, E.

AU - Halasz, M.

AU - Jericevic-Mulac, B.

AU - Wicherek, L.

AU - Arck, P.

AU - Arató, G.

AU - Skret Magierlo, J.

AU - Rukavina, D.

AU - Szekeres-Barthó, J.

PY - 2011/6

Y1 - 2011/6

N2 - Controlled trophoblast invasion is a key process during human placentation and a prerequisite for successful pregnancy. Progesterone is one of the factors to regulate trophoblast invasiveness. Progesterone-induced blocking factor (PIBF) is a progesterone-induced molecule expressed by the trophoblast, and also by tumors. The distribution of PIBF within the first-trimester decidua coincides with sites of trophoblast invasion. Another molecule that has been implicated in the control of trophoblast invasiveness is placental leptin. Leptin inhibits the secretion of progesterone by cytotrophoblast. The aim of this work was to investigate the possible interaction of PIBF and leptins in regulating trophoblast invasion. Paraffin-embedded sections from normal first-trimester placentae, partial moles, complete moles, and choriocarcinomas were reacted with PIBF, leptin, and leptin receptor specific antibodies. PIBF-deficient trophoblast cells were generated using siRNA and leptin receptor was detected on Western blot analysis. The lysates of PIBF-treated cells were used for detecting leptin expression in a protein array. PIBF was expressed in both normal first-trimester villous trophoblast and in partial mole. Compared with this, PIBF expression was markedly decreased in complete mole and absent in choriocarcinoma. Neither leptinR nor leptin were detected in partial mole, whereas both of these molecules were present in complete mole and choriocarcinoma. Leptin receptor expression was upregulated in PIBF-deficient cells, while leptin expression was decreased in PIBF-treated cells. These data suggest that PIBF affects the expression of leptin and its receptor, and that PIBF expression is inversely related to trophoblast invasiveness.

AB - Controlled trophoblast invasion is a key process during human placentation and a prerequisite for successful pregnancy. Progesterone is one of the factors to regulate trophoblast invasiveness. Progesterone-induced blocking factor (PIBF) is a progesterone-induced molecule expressed by the trophoblast, and also by tumors. The distribution of PIBF within the first-trimester decidua coincides with sites of trophoblast invasion. Another molecule that has been implicated in the control of trophoblast invasiveness is placental leptin. Leptin inhibits the secretion of progesterone by cytotrophoblast. The aim of this work was to investigate the possible interaction of PIBF and leptins in regulating trophoblast invasion. Paraffin-embedded sections from normal first-trimester placentae, partial moles, complete moles, and choriocarcinomas were reacted with PIBF, leptin, and leptin receptor specific antibodies. PIBF-deficient trophoblast cells were generated using siRNA and leptin receptor was detected on Western blot analysis. The lysates of PIBF-treated cells were used for detecting leptin expression in a protein array. PIBF was expressed in both normal first-trimester villous trophoblast and in partial mole. Compared with this, PIBF expression was markedly decreased in complete mole and absent in choriocarcinoma. Neither leptinR nor leptin were detected in partial mole, whereas both of these molecules were present in complete mole and choriocarcinoma. Leptin receptor expression was upregulated in PIBF-deficient cells, while leptin expression was decreased in PIBF-treated cells. These data suggest that PIBF affects the expression of leptin and its receptor, and that PIBF expression is inversely related to trophoblast invasiveness.

KW - Invasion

KW - Leptin

KW - PIBF

UR - http://www.scopus.com/inward/record.url?scp=79960028736&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960028736&partnerID=8YFLogxK

U2 - 10.1016/j.jri.2011.03.005

DO - 10.1016/j.jri.2011.03.005

M3 - Article

C2 - 21632119

AN - SCOPUS:79960028736

VL - 90

SP - 50

EP - 57

JO - Journal of Reproductive Immunology

JF - Journal of Reproductive Immunology

SN - 0165-0378

IS - 1

ER -