Progesterone Induced Blocking Factor Isoforms in Normal and Failed Murine Pregnancies

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Problem: Progesterone induced blocking factor (PIBF) is required for successful pregnancy. Alternative splicing produces PIBF isoforms with different functions. The full-length (90 kDa) PIBF is involved in cell cycle regulation, whereas smaller secreted forms act as cytokines. We aim to examine the PIBF exon pattern and protein isoform profile in normal and failed murine pregnancies. Method of study: Pregnant Balb/c mice were killed on gestation days 12-14 or 17-19. Normal and resorbed fetuses, placentae, and uterine tissue were used for RNA and protein analysis with RT-PCR and Western blot, respectively. Results: Late pregnancy and resorption were associated with lower expression of the N-terminal exons, together with significantly reduced production of the full-length protein. Conclusion: Reduced production of the full-length PIBF protein might result in disturbed cell cycle regulation and dysregulated trophoblast invasion, while the absence of PIBF isoforms containing exon 2-4 coded sequences might lead to the loss of local immunosuppression.

Original languageEnglish
Pages (from-to)131-136
Number of pages6
JournalAmerican Journal of Reproductive Immunology
Volume71
Issue number2
DOIs
Publication statusPublished - Feb 1 2014

Keywords

  • Exons
  • Mouse pregnancy
  • PIBF isoforms
  • Resorption

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

Fingerprint Dive into the research topics of 'Progesterone Induced Blocking Factor Isoforms in Normal and Failed Murine Pregnancies'. Together they form a unique fingerprint.

  • Cite this