Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and hCG treatment during the early luteal phase

Adam Šuluburic, Svetlana Milanovic, Sanja Vranješ-Ðuric, Ivan B. Jovanovic, Tomislav Barna, Milica Stojic, Natalija Fratric, O. Szenci, Dragan Gvozdic

Research output: Contribution to journalArticle

Abstract

Early embryonic development may be negatively affected by insufficient progesterone (P4) production. Therefore, the aim of our study was to increase P4 by gonadotropin-releasing hormone (GnRH) and/or human chorionic gonadotropin (hCG) treatments after inducing oestrus by prostaglandin (PG) treatment. Lactating Simmental dairy cows (n = 110), between 1 to 5 lactations, with an average milk production of 6,500 l/305 days, at 40-80 days postpartum were used and grouped as follows: (1) PG + GnRH treatment at AI (GnRH group), (2) PG + hCG treatment at day 7 after AI (hCG group), (3) PG + GnRH at AI + hCG treatment at day 7 after AI (GnRH/hCG group), and (4) spontaneous oestrus (C: control group). All animals were double inseminated (at the time of oestrus detection and 12 ± 2 h thereafter). Blood serum and milk samples were collected at the day of observed oestrus (day 0), and 14, 21 and 28 days after AI. Serum P4 was determined using a commercial radioimmunoassay (RIA) test (INEP, Zemun), and milk P4 was determined using enzyme-linked immunoassay (ELISA) test (NIV Novi Sad). Pregnancy status was confirmed by ultrasonography between days 28 and 35 after AI. Differences of serum or milk P4 medians, pregnancy (and calving) rate were determined using Dunn's Multiple Comparison Tests and Z test, respectively. Serum P4 medians were significantly higher at days 14, 21 and 28 after AI in the hCG-treated animals, indicating increased luteal activity, with a similar tendency in whole milk P4 values. Treatment with hCG during the early luteal phase significantly contributed to the maintenance of gestation at days 28-35 after AI, and also increased the calving rate in Simmental dairy cows.

Original languageEnglish
Pages (from-to)446-458
Number of pages13
JournalActa Veterinaria Hungarica
Volume65
Issue number3
DOIs
Publication statusPublished - Sep 1 2017

Fingerprint

Estrus Synchronization
calving rate
estrus synchronization
Luteal Phase
human chorionic gonadotropin
Simmental
Chorionic Gonadotropin
Pregnancy Rate
pregnancy rate
corpus luteum
Progesterone
progesterone
dairy cows
gonadotropin-releasing hormone
Gonadotropin-Releasing Hormone
Milk
blood serum
prostaglandins
Prostaglandins
Estrus

Keywords

  • Calving rate
  • Oestrus synchronisation
  • Pregnancy rate
  • Progesterone
  • Simmental dairy cows

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and hCG treatment during the early luteal phase. / Šuluburic, Adam; Milanovic, Svetlana; Vranješ-Ðuric, Sanja; Jovanovic, Ivan B.; Barna, Tomislav; Stojic, Milica; Fratric, Natalija; Szenci, O.; Gvozdic, Dragan.

In: Acta Veterinaria Hungarica, Vol. 65, No. 3, 01.09.2017, p. 446-458.

Research output: Contribution to journalArticle

Šuluburic, Adam ; Milanovic, Svetlana ; Vranješ-Ðuric, Sanja ; Jovanovic, Ivan B. ; Barna, Tomislav ; Stojic, Milica ; Fratric, Natalija ; Szenci, O. ; Gvozdic, Dragan. / Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and hCG treatment during the early luteal phase. In: Acta Veterinaria Hungarica. 2017 ; Vol. 65, No. 3. pp. 446-458.
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