Production of Trichoderma strains with pesticide-polyresistance by mutagenesis and protoplast fusion

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Abstract

The sensitivity of two cold-tolerant Trichoderma strains belonging to the species T. harzianum and T.atroviride was determined to a series of pesticides widely used in agriculture. From the 16 pesticides tested, seven fungicides: copper sulfate, carbendazim, mancozeb, tebuconazole, imazalil, captan and thiram inhibited colony growth of the test strains significantly with minimal inhibitory concentrations of 300, 0.4, 50, 100, 100, 100 and 50 μg/ml, respectively. Mutants resistant to carbendazim and tebuconazole were produced from both wild type strains by means of UV-mutagenesis. The cross-resistance capabilities and in vitro antagonistic properties of the mutants were determined. Carbendazim-resistant mutants showed total cross-resistance to benomyl and thiabendazole at a concentration of 20 μg/ml. Intraspecific protoplast fusion was carried out between carbendazim- and tebuconazole-resistant mutants of both parental strains, and putative haploid recombinants with stable resistance to both pesticides were produced in the case of T. atroviride. These pesticide-polyresistant progenies are potential candidates for application in an integrated pest management system.

Original languageEnglish
Pages (from-to)387-393
Number of pages7
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume89
Issue number3-4
DOIs
Publication statusPublished - May 1 2006

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Keywords

  • Biocontrol agents
  • Mutagenesis
  • Pesticide polyresistance
  • Protoplast fusion
  • Trichoderma

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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